ArticleThe Journal of physiology1999
CaM kinase II-dependent mobilization of secretory granules underlies acetylcholine-induced stimulation of exocytosis in mouse pancreatic B-cells.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion.Molecular metabolism · 2026Article
- The autonomic nervous system in the regulation of glucose and lipid metabolism.Nature reviews. Endocrinology · 2026Review
- Glucose stimulates somatostatin secretion in pancreatic δ-cells by cAMP-dependent intracellular CaThe Journal of general physiology · 2019Article
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- Application of High-Throughput Assays to Examine Phospho-Modulation of the Late Steps of Regulated Exocytosis.High-throughput · 2017Article
- Ca2+ channel clustering with insulin-containing granules is disturbed in type 2 diabetes.The Journal of clinical investigation · 2017Article
- Coadjuvants in the Diabetic Complications: Nutraceuticals and Drugs with Pleiotropic Effects.International journal of molecular sciences · 2016Review
- Changes in beta cell function occur in prediabetes and early disease in the Lepr (db) mouse model of diabetes.Diabetologia · 2016Article
- Phosphoinositide signalling in type 2 diabetes: a β-cell perspective.Biochemical Society transactions · 2016Review
- Inhibition of pancreatic β-cell Ca2+/calmodulin-dependent protein kinase II reduces glucose-stimulated calcium influx and insulin secretion, impairing glucose tolerance.The Journal of biological chemistry · 2014Article
- Article
- Regulation of insulin synthesis and secretion and pancreatic Beta-cell dysfunction in diabetes.Current diabetes reviews · 2013Review
- On depolarization-evoked exocytosis as a function of calcium entry: possibilities and pitfalls.Biophysical journal · 2011Article
- Gαo represses insulin secretion by reducing vesicular docking in pancreatic beta-cells.Diabetes · 2010Article
- BK channels mediate a novel ionic mechanism that regulates glucose-dependent electrical activity and insulin secretion in mouse pancreatic β-cells.The Journal of physiology · 2010Article
- Epac2-dependent rap1 activation and the control of islet insulin secretion by glucagon-like peptide-1.Vitamins and hormones · 2010Review
- The granular chloride channel ClC-3 is permissive for insulin secretion.Cell metabolism · 2009Article
- Unifying concepts in stimulus-secretion coupling in endocrine cells and some implications for therapeutics.Advances in physiology education · 2009Review
- Cab45b, a Munc18b-interacting partner, regulates exocytosis in pancreatic beta-cells.The Journal of biological chemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Measurements of cell capacitance were used to investigate the mechanisms by which acetylcholine (ACh) stimulates Ca2+-induced exocytosis in single insulin-secreting mouse pancreatic B-cells. 2. ACh (250 microM) increased exocytotic responses elicited by voltage-clamp depolarizations 2.3-fold. This effect was mediated by activation of muscarinic receptors and dependent on elevation of the cytoplasmic Ca2+ concentration ([Ca2+]i) attributable to mobilization of Ca2+ from intracellular stores. The latter action involved interference with the buffering of [Ca2+]i and the time constant (tau) for the recovery of [Ca2+]i following a voltage-clamp depolarization increased 5-fold. As a result, Ca2+ was present at concentrations sufficient to promote the replenishment of the readily releasable pool of granules (RRP; > 0.2 microM) for much longer periods in the presence than in the absence of the agonist. 3. The effect of Ca2+ on exocytosis was mediated by activation of CaM kinase II, but not protein kinase C, and involved both an increased size of the RRP from 40 to 140 granules and a decrease in tau for the refilling of the RRP from 31 to 19 s. 4. Collectively, the effects of ACh on the RRP and tau result in a > 10-fold stimulation of the rate at which granules are supplied for release.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.