ArticleMolecular and cellular biology1999
Identification of cis and trans elements involved in the cell cycle regulation of multiple genes in Crithidia fasciculata.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Extensive Translational Regulation through the Proliferative Transition of Trypanosoma cruzi Revealed by Multi-Omics.mSphere · 2021Article
- Gene co-expression network analysis of Trypanosoma brucei in tsetse fly vector.Parasites & vectors · 2021Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- High-throughput Methods for Dissection of Trypanosome Gene Regulatory Networks.Current genomics · 2018Review
- A short RNA stem-loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes.Nucleic acids research · 2014Article
- The mRNAs associated to a zinc finger protein from Trypanosoma cruzi shift during stress conditions.RNA biology · 2014Article
- Trans-acting proteins regulating mRNA maturation, stability and translation in trypanosomatids.Trends in parasitology · 2011Review
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- Genome-wide in silico screen for CCCH-type zinc finger proteins of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major.BMC genomics · 2010Article
- A mitochondrial DNA primase is essential for cell growth and kinetoplast DNA replication in Trypanosoma brucei.Molecular and cellular biology · 2010Article
- Coordinate regulation of a family of promastigote-enriched mRNAs by the 3'UTR PRE element in Leishmania mexicana.Molecular and biochemical parasitology · 2008Article
- Identification of new kinetoplast DNA replication proteins in trypanosomatids based on predicted S-phase expression and mitochondrial targeting.Eukaryotic cell · 2007Article
- Cell cycle-dependent localization and properties of a second mitochondrial DNA ligase in Crithidia fasciculata.Eukaryotic cell · 2006Article
- A novel strategy to identify the location of necessary and sufficient cis-acting regulatory mRNA elements in trypanosomes.RNA (New York, N.Y.) · 2005Article
- Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements.Nucleic acids research · 2005Article
- Presence of a poly(A) binding protein and two proteins with cell cycle-dependent phosphorylation in Crithidia fasciculata mRNA cycling sequence binding protein II.Eukaryotic cell · 2004Article
- Expression of exogenous genes in Trypanosoma cruzi: improving vectors and electroporation protocols.Parasitology research · 2004Article
- A negative regulatory element controls mRNA abundance of the Leishmania mexicana Paraflagellar rod gene PFR2.Eukaryotic cell · 2003Article
- Cloning, functional analysis and post-transcriptional regulation of a type II DNA topoisomerase from Leishmania infantum. A new potential target for anti-parasite drugs.Nucleic acids research · 2003Article
- Sequence elements in both the intergenic space and the 3' untranslated region of the Crithidia fasciculata KAP3 gene are required for cell cycle regulation of KAP3 mRNA.Eukaryotic cell · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transcripts of several DNA replication genes, including the RPA1 and TOP2 genes, encoding the large subunit of nuclear replication protein A and the kinetoplast topoisomerase II, accumulate periodically during the cell cycle in the trypanosomatid Crithidia fasciculata. An octamer consensus sequence, CAUAGAAG, present in the 5' untranslated regions (UTR) of these mRNAs is required for periodic accumulation of the TOP2 and RPA1 transcripts and also for binding of a nuclear factor(s) to the 5' UTR RNAs of these genes. We show here that insertion of multiple (six) copies of this octamer sequence (6x octamer) into the 5' UTR of a reporter gene confers periodic accumulation on its transcript. Competition experiments and UV cross-linking studies show that the 6x octamer RNA and TOP2 5' UTR RNA bind to the same nuclear factor(s). Single-nucleotide substitutions in the 6x octamer that abolish the RNA gel shift also prevent cyclic accumulation of the reporter gene transcript. A protein termed cycling element binding protein, purified by affinity chromatography using 6x octamer RNA as a ligand, binds to RNAs containing wild-type octamers and not to those with mutant octamers. These results define a small sequence element in C. fasciculata mRNAs required for their cell cycle regulation and report the identification and purification of a putative regulatory protein that binds specifically to these elements.
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