ArticleThe Biochemical journal2000
Activation of exocytosis by cross-linking of the IgE receptor is dependent on ADP-ribosylation factor 1-regulated phospholipase D in RBL-2H3 mast cells: evidence that the mechanism of activation is via regulation of phosphatidylinositol 4,5-bisphosphate synthesis.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- Antimicrobial agent triclosan suppresses mast cell signaling via phospholipase D inhibition.Journal of applied toxicology : JAT · 2019Article
- The multifaceted role of PIP2 in leukocyte biology.Cellular and molecular life sciences : CMLS · 2015Review
- Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization.Developmental biology · 2014Article
- Phospholipase D2: a pivotal player modulating RBL-2H3 mast cell structure.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Article
- Phosphoinositide phosphatases in cell biology and disease.Progress in lipid research · 2010Review
- Lipid second messengers and related enzymes in vertebrate rod outer segments.Journal of lipid research · 2010Review
- Phospholipase d promotes lipid microdomain-associated signaling events in mast cells.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- An intracellular motif of GLUT4 regulates fusion of GLUT4-containing vesicles.BMC cell biology · 2008Article
- Phospholipase D signaling: orchestration by PIP2 and small GTPases.Naunyn-Schmiedeberg's archives of pharmacology · 2007Review
- Article
- PldB, a putative phospholipase D homologue in Dictyostelium discoideum mediates quorum sensing during development.Eukaryotic cell · 2005Article
- Activation of RBL-2H3 mast cells is dependent on tyrosine phosphorylation of phospholipase D2 by Fyn and Fgr.Molecular and cellular biology · 2004Article
- Arf-1 (ADP-ribosylation factor-1) is involved in the activation of a mammalian Na+-selective current.The Biochemical journal · 2004Article
- Regulation of phospholipase D1 subcellular cycling through coordination of multiple membrane association motifs.The Journal of cell biology · 2003Article
- Endogenous phospholipase D2 localizes to the plasma membrane of RBL-2H3 mast cells and can be distinguished from ADP ribosylation factor-stimulated phospholipase D1 activity by its specific sensitivity to oleic acid.The Biochemical journal · 2003Article
- Localization of phospholipase D1 to caveolin-enriched membrane via palmitoylation: implications for epidermal growth factor signaling.Molecular biology of the cell · 2002Article
- Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells.Molecular biology of the cell · 2002Article
- Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells.Molecular biology of the cell · 2002Article
- Cell-permeable ceramides preferentially inhibit coated vesicle formation and exocytosis in Chinese hamster ovary compared with Madin-Darby canine kidney cells by preventing the membrane association of ADP-ribosylation factor.The Biochemical journal · 2002Article
- Activated Cdc42/Rac reconstitutes Fcepsilon RI-mediated Ca2+ mobilization and degranulation in mutant RBL mast cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The physiological stimulus to exocytosis in mast cells is the cross-linking of the high-affinity IgE receptor, FcepsilonR1, with antigen. We demonstrate a novel function for ADP-ribosylation factor 1 (ARF1) in the regulation of antigen-stimulated secretion using cytosol-depleted RBL-2H3 mast cells for reconstitution of secretory responses. When antigen is used as the stimulus, ARF1 also reconstitutes phospholipase D activation. Using ethanol to divert the phosphatidic acid (the product of phospholipase D activity) to phosphatidylethanol causes inhibition of ARF1-reconstituted secretion. In addition. ARF1 causes an increase in phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels at the expense of phosphatidylinositol 4-monophosphate. The requirement for PIP(2) in exocytosis was confirmed by using phosphatidylinositol transfer protein (PITPalpha) to increase PIP(2) levels. Exocytosis, restored by either ARF1 or PITPalpha, was inhibited when PIP(2) levels were depleted by phospholipase Cdelta1. We conclude that the function of ARF1 and PITPalpha is to increase the local synthesis of PIP(2), the function of which in exocytosis is likely to be linked to lipid-protein interactions, whereby recruitment of key components of the exocytotic machinery are targeted to the appropriate membrane compartment.
Indexed as
Identifiers
10657240PMC1220823W2099036383What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.