Evidence map›Paper›PMID 10899111›Full record

ArticleThe EMBO journal2000

Discrimination of GLUT4 vesicle trafficking from fusion using a temperature-sensitive Munc18c mutant.

D C Thurmond, J E Pessin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
6.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 54 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Exocytosis mechanisms underlying insulin release and glucose uptake: conserved roles for Munc18c and syntaxin 4.American journal of physiology. Regulatory, integrative and comparative physiology · 2010
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Fluidity of insulin action.Molecular biotechnology · 2004
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

D C ThurmondDepartment of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242-1109, USA.
J E Pessin
University of Iowa · US

Funding

REGULATION OF THE INSULIN RECEPTOR KINASER01DK033823 · NIDDK · UNIVERSITY OF IOWA · PI KURLAND, IRWIN JACK, PESSIN, JEFFREY E. · 1986 to 2022
$6.7M
Intracellular signaling by the insulin receptor kinaseR37DK033823 · NIDDK · STATE UNIVERSITY NEW YORK STONY BROOK · PI PESSIN, JEFFREY E. · 2005 to 2014
$4.2M
REGULATION OF INSULIN STIMULATED GLUT4 TRANSLOCATIONF32DK009813 · NIDDK · UNIVERSITY OF IOWA · PI THURMOND, DEBBIE C · 1998 to 1999
–
NIDDK NIH HHS DK09813NIDDK NIH HHS DK25925NIDDK NIH HHS DK33823NIDDK NIH HHS F32 DK009813NIDDK NIH HHS R01 DK033823NIDDK NIH HHS R37 DK033823
6 · The paper itself

Abstract

To examine the temporal relationship between pre- and post-docking events, we generated a Munc18c temperature-sensitive mutant (Munc18c/TS) by substitution of arginine 240 with a lysine residue. At the permissive temperature (23 degrees C), overexpression of both the wild type (Munc18c/WT) and the R240K mutant inhibited insulin-stimulated GLUT4/IRAP vesicle translocation. However, at the non-permissive temperature (37 degrees C) only Munc18c/WT inhibited GLUT4/IRAP translocation whereas Munc18c/TS was without effect. Moreover, Munc18c/WT bound to syntaxin 4 at both 23 and 37 degrees C whereas Munc18c/TS bound syntaxin 4 only at 23 degrees C. This was due to a temperature-dependent conformational change in Munc18c/TS, as its ability to bind syntaxin 4 and effects on GLUT4 translocation were rapidly reversible while protein expression levels remained unchanged. Furthermore, insulin stimulation of Munc18c/TS-expressing cells at 23 degrees C followed by temperature shift to 37 degrees C resulted in an increased rate of GLUT4 translocation compared with cells stimulated at 37 degrees C. To date, this is the first demonstration that the rate-limiting step for insulin-stimulated GLUT4 translocation is the trafficking of GLUT4 vesicles and not their fusion with the plasma membrane.

Indexed as

Membrane FusionMuscle ProteinsMutationNerve Tissue ProteinsVesicular Transport Proteins3T3 CellsAdipocytesAmino Acid SequenceAmino Acid SubstitutionAminopeptidasesAnimalsArginineBiological TransportCell MembraneCystinyl AminopeptidaseGlucose Transporter Type 4AminopeptidasesArginineCystinyl AminopeptidaseGlucose Transporter Type 4Insulinleucyl-cystinyl aminopeptidaseLysineMembrane ProteinsMonosaccharide Transport ProteinsMunc18 ProteinsMuscle ProteinsNerve Tissue ProteinsProteinsQa-SNARE ProteinsReceptor, IGF Type 2Slc2a4 protein, mouseStxbp3 protein, mouseVesicular Transport Proteins

Identifiers

PMID10899111
PMCPMC313977
OpenAlexW1975522394

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.