Evidence map›Paper›PMID 10934247›Full record

ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000

The Ets transcription factor GABP is required for postsynaptic differentiation in vivo.

A Briguet, M A Ruegg

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 76 citations in OpenAlex.

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  7. Modeling the transport of nuclear proteins along single skeletal muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 2020
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  11. Molecular control of neuromuscular junction development.Journal of cachexia, sarcopenia and muscle · 2012
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  19. Organization of synaptic myonuclei by Syne proteins and their role during the formation of the nerve-muscle synapse.Proceedings of the National Academy of Sciences of the United States of America · 2005
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

A BriguetDepartment of Pharmacology/Neurobiology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland.
M A Ruegg
University of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

At chemical synapses, neurotransmitter receptors are concentrated in the postsynaptic membrane. During the development of the neuromuscular junction, motor neurons induce aggregation of acetylcholine receptors (AChRs) underneath the nerve terminal by the redistribution of existing AChRs and preferential transcription of the AChR subunit genes in subsynaptic myonuclei. Neural agrin, when expressed in nonsynaptic regions of muscle fibers in vivo, activates both mechanisms resulting in the assembly of a fully functional postsynaptic apparatus. Several lines of evidence indicate that synaptic transcription of AChR genes is primarily dependent on a promoter element called N-box. The Ets-related transcription factor growth-associated binding protein (GABP) binds to this motif and has thus been suggested to regulate synaptic gene expression. Here, we assessed the role of GABP in synaptic gene expression and in the formation of postsynaptic specializations in vivo by perturbing its function during postsynaptic differentiation induced by neural agrin. We find that neural agrin-mediated activation of the AChR epsilon subunit promoter is abolished by the inhibition of GABP function. Importantly, the number of AChR aggregates formed in response to neural agrin was strongly reduced. Moreover, aggregates of acetylcholine esterase and utrophin, two additional components of the postsynaptic apparatus, were also reduced. Together, these results are the first direct in vivo evidence that GABP regulates synapse-specific gene expression at the neuromuscular junction and that GABP is required for the formation of a functional postsynaptic apparatus.

Indexed as

AcetylcholinesteraseAgrinAnimalsCell DifferentiationCOS CellsCytoskeletal ProteinsDNA-Binding ProteinsGA-Binding Protein Transcription FactorGene Expression Regulation, DevelopmentalMembrane ProteinsMuscle Fibers, SkeletalMutationNeuromuscular JunctionPromoter Regions, GeneticRatsReceptors, CholinergicAcetylcholinesteraseAgrinCytoskeletal ProteinsDNA-Binding ProteinsGA-Binding Protein Transcription FactorMembrane ProteinsReceptors, CholinergicTranscription FactorsUtrophin

Identifiers

PMID10934247
PMCPMC6772583
OpenAlexW2129970296

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.