ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Nerve growth factor, but not epidermal growth factor, increases Fra-2 expression and alters Fra-2/JunD binding to AP-1 and CREB binding elements in pheochromocytoma (PC12) cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- The Differential Effects of Repetitive Magnetic Stimulation in anFrontiers in neurology · 2018Article
- A c-Jun N-terminal kinase inhibitor, JNK-IN-8, sensitizes triple negative breast cancer cells to lapatinib.Oncotarget · 2017Article
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- Modulation of neurotrophic signaling pathways by polyphenols.Drug design, development and therapy · 2016Review
- Transcriptional and epigenetic substrates of methamphetamine addiction and withdrawal: evidence from a long-access self-administration model in the rat.Molecular neurobiology · 2015Review
- Follistatin in chondrocytes: the link between TRPV4 channelopathies and skeletal malformations.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Progressive accumulation of activated ERK2 within highly stable ORF45-containing nuclear complexes promotes lytic gammaherpesvirus infection.PLoS pathogens · 2014Article
- Neurotrophic effect of citrus auraptene: neuritogenic activity in PC12 cells.International journal of molecular sciences · 2012Article
- Luteolin induces microRNA-132 expression and modulates neurite outgrowth in PC12 cells.PloS one · 2012Article
- Global expression analysis identified a preferentially nerve growth factor-induced transcriptional program regulated by sustained mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) and AP-1 protein activation during PC12 cell differentiation.The Journal of biological chemistry · 2011Article
- Selective genomic targeting by FRA-2/FOSL2 transcription factor: regulation of the Rgs4 gene is mediated by a variant activator protein 1 (AP-1) promoter sequence/CREB-binding protein (CBP) mechanism.The Journal of biological chemistry · 2011Article
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- Critical role for Kalirin in nerve growth factor signaling through TrkA.Molecular and cellular biology · 2005Article
- The transcriptional response to hypoxic insult controlled by FRA-2.Gene expression · 2005Article
- Expression profiling upon Nex1/MATH-2-mediated neuritogenesis in PC12 cells and its implication in regeneration.Journal of neurochemistry · 2004Article
- A dominant negative Egr inhibitor blocks nerve growth factor-induced neurite outgrowth by suppressing c-Jun activation: role of an Egr/c-Jun complex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- AP-1 proteins in the adult brain: facts and fiction about effectors of neuroprotection and neurodegeneration.Oncogene · 2001Review
- BMP enhances transcriptional responses to NGF during PC12 cell differentiation.Neurochemical researchArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In pheochromocytoma (PC12) cells nerve growth factor (NGF) and epidermal growth factor (EGF) activate similar receptor tyrosine kinase signaling pathways but evoke strikingly different biological outcomes: NGF induces differentiation and EGF acts as a mitogen. A novel approach was developed for identifying transcription factor activities associated with NGF-activated, but not EGF-activated, signaling, using random oligonucleotide clones from a DNA recognition library to isolate specific DNA binding proteins from PC12 nuclear extracts. A protein complex from NGF-treated, but not EGF-treated, cells was identified that exhibits increased mobility and DNA binding activity in gel mobility shift assays. The binding complex was identified in supershift assays as Fra-2/JunD. The clones used as probes contain either AP-1 or cAMP response element binding (CREB) recognition elements. Time course experiments revealed further differences in NGF and EGF signaling in PC12 cells. NGF elicits a more delayed and sustained ERK phosphorylation than EGF, consistent with previous reports. Both growth factors transiently induce c-fos, but NGF evokes a greater response than EGF. NGF specifically increases Fra-1 and Fra-2 levels at 4 and 24 hr. The latter is represented in Western blots by bands in the 40-46 kDa range. NGF, but not EGF, enhances the upper bands, corresponding to phosphorylated Fra-2. These findings suggest that prolonged alterations in Fra-2 and subsequent increases in Fra-2/JunD binding to AP-1 and CREB response elements common among many gene promoters could serve to trigger broadly an NGF-specific program of gene expression.
Indexed as
Identifiers
11150315PMC6762456W1911828269What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.