ArticleThe Journal of cell biology2001
A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 148 citations in OpenAlex.
- Arp2/3-mediated bidirectional actin assembly by SPIN90 dimers.Nature structural & molecular biology · 2025Article
- Small GTPase Cdc42, WASP, and scaffold proteins for higher-order assembly of the F-BAR domain protein.Science advances · 2023Article
- Analysis of functional surfaces on the actin nucleation promoting factor Dip1 required for Arp2/3 complex activation and endocytic actin network assembly.The Journal of biological chemistry · 2022Article
- A Subtle Network Mediating Axon Guidance: Intrinsic Dynamic Structure of Growth Cone, Attractive and Repulsive Molecular Cues, and the Intermediate Role of Signaling Pathways.Neural plasticity · 2019Review
- Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.The EMBO journal · 2018Article
- New insights into the formation and the function of lamellipodia and ruffles in mesenchymal cell migration.Cell adhesion & migration · 2018Review
- Molecular and cellular impact of Psoriasin (S100A7) on the healing of human wounds.Experimental and therapeutic medicine · 2017Article
- A role for the small GTPase Rac1 in vaccinia actin-based motility.Small GTPases · 2015Review
- Steering cell migration: lamellipodium dynamics and the regulation of directional persistence.Nature reviews. Molecular cell biology · 2014Review
- A role for the small GTPase Rac1 in vaccinia actin-based motility.Small GTPases · 2014Article
- Dip1 defines a class of Arp2/3 complex activators that function without preformed actin filaments.Current biology : CB · 2013Article
- The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility.The Journal of cell biology · 2013Article
- Surfing the big WAVE: Insights into the role of WAVE3 as a driving force in cancer progression and metastasis.Seminars in cell & developmental biology · 2013Review
- Under lock and key: spatiotemporal regulation of WASP family proteins coordinates separate dynamic cellular processes.Seminars in cell & developmental biology · 2013Review
- Stoichiometry of Nck-dependent actin polymerization in living cells.The Journal of cell biology · 2012Article
- Protein and phosphoprotein levels in glioma and adenocarcinoma cell lines grown in normoxia and hypoxia in monolayer and three-dimensional cultures.Proteome science · 2012Article
- Dia-interacting protein (DIP) imposes migratory plasticity in mDia2-dependent tumor cells in three-dimensional matrices.PloS one · 2012Article
- Characterization of dip1p reveals a switch in Arp2/3-dependent actin assembly for fission yeast endocytosis.Current biology : CB · 2011Article
- Cdc42-interacting protein 4 promotes breast cancer cell invasion and formation of invadopodia through activation of N-WASp.Cancer research · 2010Article
- Cytoskeletal dynamics and spermatogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We identified a novel adaptor protein that contains a Src homology (SH)3 domain, SH3 binding proline-rich sequences, and a leucine zipper-like motif and termed this protein WASP interacting SH3 protein (WISH). WISH is expressed predominantly in neural tissues and testis. It bound Ash/Grb2 through its proline-rich regions and neural Wiskott-Aldrich syndrome protein (N-WASP) through its SH3 domain. WISH strongly enhanced N-WASP-induced Arp2/3 complex activation independent of Cdc42 in vitro, resulting in rapid actin polymerization. Furthermore, coexpression of WISH and N-WASP induced marked formation of microspikes in Cos7 cells, even in the absence of stimuli. An N-WASP mutant (H208D) that cannot bind Cdc42 still induced microspike formation when coexpressed with WISH. We also examined the contribution of WISH to a rapid actin polymerization induced by brain extract in vitro. Arp2/3 complex was essential for brain extract-induced rapid actin polymerization. Addition of WISH to extracts increased actin polymerization as Cdc42 did. However, WISH unexpectedly could activate actin polymerization even in N-WASP-depleted extracts. These findings suggest that WISH activates Arp2/3 complex through N-WASP-dependent and -independent pathways without Cdc42, resulting in the rapid actin polymerization required for microspike formation.
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