ArticleThe Journal of cell biology2001
Vps34p differentially regulates endocytosis from the apical and basolateral domains in polarized hepatic cells.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- PAR3-mediated coordination of hepatocyte proliferation, maturation, and architecture in liver development and regeneration.Nature communications · 2026Article
- Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathy.The Journal of clinical investigation · 2023Article
- Using Phosphatidylinositol Phosphorylation as Markers for Hyperglycemic Related Breast Cancer.International journal of molecular sciences · 2020Article
- Rab17 regulates apical delivery of hepatic transcytotic vesicles.Molecular biology of the cell · 2018Article
- The LTBJournal of cell science · 2018Article
- Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins.Nature communications · 2018Article
- A Serine/Threonine Kinase 16-Based Phospho-Proteomics Screen Identifies WD Repeat Protein-1 As A Regulator Of Constitutive Secretion.Scientific reports · 2018Article
- Copper directs ATP7B to the apical domain of hepatic cells via basolateral endosomes.Traffic (Copenhagen, Denmark) · 2014Article
- Importance of endocytic pathways in liver function and disease.Comprehensive Physiology · 2014Review
- Cytokinesis defines a spatial landmark for hepatocyte polarization and apical lumen formation.Journal of cell science · 2014Article
- Ursodeoxycholic acid stabilizes the bile salt export pump in the apical membrane in MDCK II cells.Journal of gastroenterology · 2014Article
- Membrane protein location-dependent regulation by PI3K (III) and rabenosyn-5 in Drosophila wing cells.PloS one · 2009Article
- In vitro intracellular trafficking of virulence antigen during infection by Yersinia pestis.PloS one · 2009Article
- Effect of ethanol on pro-apoptotic mechanisms in polarized hepatic cells.World journal of gastroenterology · 2007Review
- Recycling endosomes of polarized epithelial cells actively sort apical and basolateral cargos into separate subdomains.Molecular biology of the cell · 2007Article
- Anchoring of protein kinase A-regulatory subunit IIalpha to subapically positioned centrosomes mediates apical bile canalicular lumen development in response to oncostatin M but not cAMP.Molecular biology of the cell · 2007Article
- The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane.Molecular biology of the cell · 2005Article
- Intracellular trafficking of bile salt export pump (ABCB11) in polarized hepatic cells: constitutive cycling between the canalicular membrane and rab11-positive endosomes.Molecular biology of the cell · 2004Article
- Nonpolarized cells selectively sort apical proteins from cell surface to a novel compartment, but lack apical retention mechanisms.Molecular biology of the cell · 2002Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Using a microinjection approach to study apical plasma membrane protein trafficking in hepatic cells, we found that specific inhibition of Vps34p, a class III phosphoinositide 3 (PI-3) kinase, nearly perfectly recapitulated the defects we reported for wortmannin-treated cells (Tuma, P.L., C.M. Finnegan, J.-H Yi, and A.L. Hubbard. 1999. J. Cell Biol. 145:1089-1102). Both wortmannin and injection of inhibitory Vps34p antibodies led to the accumulation of resident apical proteins in enlarged prelysosomes, whereas transcytosing apical proteins and recycling basolateral receptors transiently accumulated in basolateral early endosomes. To understand how the Vps34p catalytic product, PI3P, was differentially regulating endocytosis from the two domains, we examined the PI3P binding protein early endosomal antigen 1 (EEA1). We determined that EEA1 distributed to two biochemically distinct endosomal populations: basolateral early endosomes and subapical endosomes. Both contained rab5, although the latter also contained late endosomal markers but was distinct from the transcytotic intermediate, the subapical compartment. When PI3P was depleted, EEA1 dissociated from basolateral endosomes, whereas it remained on subapical endosomes. From these results, we conclude that PI3P, via EEA1, regulates early steps in endocytosis from the basolateral surface in polarized WIF-B cells. However, PI3P must use different machinery in its regulation of the apical endocytic pathway, since later steps are affected by Vps34p inhibition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.