ArticleJournal of molecular neuroscience : MN2002
A quantitative bioassay for nerve growth factor, using PC12 clones expressing different levels of trkA receptors.
Article in Journal of molecular neuroscience : MN, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Neurotropic activity and safety of methylene-cycloalkylacetate (MCA) derivative 3-(3-allyl-2-methylenecyclohexyl) propanoic acid.ACS chemical neuroscience · 2020Article
- Tuftelin Is Required for NGF-Induced Differentiation of PC12 Cells.Journal of molecular neuroscience : MN · 2019Article
- Transcriptional down-regulation of epidermal growth factor (EGF) receptors by nerve growth factor (NGF) in PC12 cells.Journal of molecular neuroscience : MN · 2014Review
- Nanofibrous scaffold with incorporated protein gradient for directing neurite outgrowth.Drug delivery and translational research · 2011Article
- A rapid, inexpensive high throughput screen method for neurite outgrowth.Current chemical genomics · 2010Article
- Angiostatic effects of K252a, a Trk inhibitor, in murine brain capillary endothelial cells.Molecular and cellular biochemistry · 2010Article
- Cardiac microvascular endothelial cells express and release nerve growth factor but not fibroblast growth factor-2.In vitro cellular & developmental biology. Animal · 2010Article
- Interferon-gamma-induced neuronal differentiation of human umbilical cord blood-derived progenitors.Leukemia · 2009Article
- Quantitative assessment of neuronal differentiation in three-dimensional collagen gels using enhanced green fluorescence protein expressing PC12 pheochromocytoma cells.Journal of molecular neuroscience : MN · 2009Article
- Neuronal conditioning medium and nerve growth factor induce neuronal differentiation of collagen-adherent progenitors derived from human umbilical cord blood.Journal of molecular neuroscience : MN · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nerve growth factor (NGF) is a neurotrophin required for differentiation, development, and survival of the sympathetic nervous system, with many of its biological effects being mediated via trkA receptors. There is a need for a standard quantitative bioassay for NGF, to be used in basic research and in pharmaceutical studies. The objective of the present research was to develop a selective, quantitative, and reliable bioassay for NGF, using a morphological criterion: neurite cell outgrowth. In addition, we aimed to apply the aforementioned bioassay to measure NGF administered to mice. Pheochromocytoma PC12 cell variants including wild-type cultures, and a trkA-overexpressing stable transfectant PC12-6.24-I, PC12nnr5, and PC12EN lacking trkA receptors, were used. Dose-response curves were generated with NGF beta-subunit (2.5S) purified from mouse submaxillary glands. Our results demonstrated that the bioassay was sensitive to 0.3-20 ng/mL, and selective, as neurite outgrowth was not seen by any other growth factor other than NGF. In addition, variant clones PC12nnr5 and PC12EN, lacking trkA receptors, did not respond to NGF. The bioassay detected NGF in serum of mice injected with NGF. This novel developed bioassay can serve as a model system for various neuroscience purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.