ArticleLipids2002
Identification and expression of mammalian long-chain PUFA elongation enzymes.
Article in Lipids, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 214 citations in OpenAlex.
- Polyunsaturated fatty acid metabolism in the retinal pigment epithelium and its association with outer retinal disease.Mammalian genome : official journal of the International Mammalian Genome Society · 2026Review
- From the lab to lifestyle: epigenetic clocks in personalized aging and health.Biogerontology · 2026Review
- The acyl- and alkyl-glycerone phosphate reductase DHRS7 is involved in the production of distinct plasmalogen species from DHRS7B.Journal of cell science · 2026Article
- A workflow to explore elongase diversity and extend the repertoire of fatty acids produced by Yarrowia lipolytica.Microbial cell factories · 2025Article
- Retinal polyunsaturated fatty acid supplementation reverses aging-related vision decline in mice.Science translational medicine · 2025Article
- A Sex-Specific Minimal CpG-Based Model for Biological Aging UsingInternational journal of molecular sciences · 2025Article
- Association Between Dietary Habits and the Semen Quality of South Asian Men Attending Fertility Clinic: A Cross-sectional Study.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment.bioRxiv : the preprint server for biology · 2024Article
- Polyunsaturated Fatty Acid - mediated Cellular Rejuvenation for Reversing Age-related Vision Decline.bioRxiv : the preprint server for biology · 2024Article
- Contribution ofAnimals : an open access journal from MDPI · 2024Article
- ZebrafishFrontiers in cell and developmental biology · 2024Article
- Loss of carnitine palmitoyltransferase 1a reduces docosahexaenoic acid-containing phospholipids and drives sexually dimorphic liver disease in mice.Molecular metabolism · 2023Article
- HSD17B4 methylation enhances glucose dependence of BT-474 breast cancer cells and increases lapatinib sensitivity.Breast cancer research and treatment · 2023Article
- The NAD salvage pathway in mesenchymal cells is indispensable for skeletal development in mice.Nature communications · 2023Article
- Review
- Therapeutic Role of ELOVL in Neurological Diseases.ACS omega · 2023Review
- Review
- ELOVL gene family plays a virtual role in response to breeding selection and lipid deposition in different tissues in chicken (Gallus gallus).BMC genomics · 2022Article
- Glioblastoma Multiforme Tumors in Women Have a Lower Expression of Fatty Acid ElongasesBrain sciences · 2022Article
- A chromosome-level genome assembly of the jade perch (Scortum barcoo).Scientific data · 2022Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian cells, Sprecher has proposed that the synthesis of long-chain PUFA from the 20-carbon substrates involves two consecutive elongation steps, a delta6-desaturation step followed by retroconversion (Sprecher, H., Biochim. Biophys. Acta 1486, 219-231, 2000). We searched the database using the translated sequence of human elongase ELOVL5, whose encoded enzyme elongates monounsaturated and polyunsaturated FA, as a query to identify the enzyme(s) involved in elongation of very long chain PUFA. The database search led to the isolation of two cDNA clones from human and mouse. These clones displayed deduced amino acid sequences that had 56.4 and 58% identity, respectively, to that of ELOVL5. The open reading frame of the human clone (ELOVL2) encodes a 296-amino acid peptide, whereas the mouse clone (Elovl2) encodes a 292-amino acid peptide. Expression of these open reading frames in baker's yeast, Saccharomyces cerevisiae, demonstrated that the encoded proteins were involved in the elongation of both 20- and 22-carbon long-chain PUFA, as determined by the conversion of 20:4n-6 to 22:4n-6, 22:4n-6 to 24:4n-6, 20:5n-3 to 22:5n-3, and 22:5n-3 to 24:5n-3. The elongation activity of the mouse Elovl2 was further demonstrated in the transformed mouse L cells incubated with long-chain (C20- and C22-carbon) n-6 and n-3 PUFA substrates by the significant increase in the levels of 24:4n-6 and 24:5n-3, respectively. This report demonstrates the isolation and identification of two mammalian genes that encode very long chain PUFA specific elongation enzymes in the Sprecher pathway for DHA synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.