ArticleMolecular biology of the cell2002
Nonpolarized cells selectively sort apical proteins from cell surface to a novel compartment, but lack apical retention mechanisms.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Direct trafficking pathways from the Golgi apparatus to the plasma membrane.Seminars in cell & developmental biology · 2020Review
- MAL2-Induced Actin-Based Protrusion Formation is Anti-Oncogenic in Hepatocellular Carcinoma.Cancers · 2020Article
- Rab17 regulates apical delivery of hepatic transcytotic vesicles.Molecular biology of the cell · 2018Article
- Myosin Vb mediates Cu+ export in polarized hepatocytes.Journal of cell science · 2016Article
- Sex Hormone Binding Globulin Modifies Testosterone Action and Metabolism in Prostate Cancer Cells.International journal of endocrinology · 2016Article
- Alcohol-induced defects in hepatic transcytosis may be explained by impaired dynein function.Molecular and cellular biochemistry · 2014Article
- The unique polarity phenotype of hepatocytes.Experimental cell research · 2014Review
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Analysis of polarized membrane traffic in hepatocytes and hepatic cell lines.Current protocols in cell biology · 2012Article
- AKAP350 Is involved in the development of apical "canalicular" structures in hepatic cells HepG2.Journal of cellular physiology · 2012Article
- MAL2 selectively regulates polymeric IgA receptor delivery from the Golgi to the plasma membrane in WIF-B cells.Traffic (Copenhagen, Denmark) · 2010Article
- Rab11b regulates the apical recycling of the cystic fibrosis transmembrane conductance regulator in polarized intestinal epithelial cells.Molecular biology of the cell · 2009Article
- Vectorial entry and release of hepatitis A virus in polarized human hepatocytes.Journal of virology · 2008Article
- Effect of ethanol on pro-apoptotic mechanisms in polarized hepatic cells.World journal of gastroenterology · 2007Review
- Quantitative analysis of membrane remodeling at the phagocytic cup.Molecular biology of the cell · 2007Article
- Exogenous MAL reroutes selected hepatic apical proteins into the direct pathway in WIF-B cells.Molecular biology of the cell · 2007Article
- Par1b promotes hepatic-type lumen polarity in Madin Darby canine kidney cells via myosin II- and E-cadherin-dependent signaling.Molecular biology of the cell · 2007Article
- Par-1 promotes a hepatic mode of apical protein trafficking in MDCK cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Mammalian PAR-1 determines epithelial lumen polarity by organizing the microtubule cytoskeleton.The Journal of cell biology · 2004Article
- Heterotrimeric G protein subunits are located on rat liver endosomes.BMC physiology · 2004Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Membrane trafficking is central to establishing and maintaining epithelial cell polarity. One open question is to what extent the mechanisms regulating membrane trafficking are conserved between nonpolarized and polarized cells. To answer this question, we examined the dynamics of domain-specific plasma membrane (PM) proteins in three classes of hepatic cells: polarized and differentiated WIF-B cells, nonpolarized and differentiated Fao cells, and nonpolarized and nondifferentiated Clone 9 cells. In nonpolarized cells, mature apical proteins were uniformly distributed in the PM. Surprisingly, they were also in an intracellular compartment. Double labeling revealed that the compartment contained only apical proteins. By monitoring the dynamics of antibody-labeled molecules in nonpolarized cells, we further found that apical proteins rapidly recycled between the compartment and PM. In contrast, the apical PM residents in polarized cells showed neither internalization nor return to the basolateral PM from which they had originally come. Cytochalasin D treatment of these polarized cells revealed that the retention mechanisms are actin dependent. We conclude from these data that both polarized and nonpolarized cells selectively sort apical proteins from the PM and transport them to specific, but different cellular locations. We propose that the intracellular recycling compartment in nonpolarized cells is an intermediate in apical surface formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.