ArticleCardiovascular research2002
Physiological concentrations of insulin induce endothelin-mediated vasoconstriction during inhibition of NOS or PI3-kinase in skeletal muscle arterioles.
Article in Cardiovascular research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03883412 (Effect of Exercise and/or Liraglutide on Vascular Dysfunction and Insulin Sensitivity in Type 2 Diabetes), which is not on this map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Exercise and/or Liraglutide on Vascular Dysfunction and Insulin Sensitivity in Type 2 Diabetes ( ZQL007)
Who cites it
52 citing papers in PubMed, 116 citations in OpenAlex.
- Sugar-sweetened soft drink consumption acutely decreases spontaneous baroreflex sensitivity and heart rate variability.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Trial
- Early microvascular recruitment modulates subsequent insulin-mediated skeletal muscle glucose metabolism during lipid infusion.Diabetes care · 2013 · on this mapTrial
- Seven days of aerobic exercise training improves conduit artery blood flow following glucose ingestion in patients with type 2 diabetes.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Trial
- Meal-related increases in microvascular vasomotion are impaired in obese individuals: a potential mechanism in the pathogenesis of obesity-related insulin resistance.Diabetes care · 2011Trial
- Free fatty acids induce insulin resistance in both cardiac and skeletal muscle microvasculature in humans.The Journal of clinical endocrinology and metabolism · 2011 · on this mapTrial
- Hyperinsulinemia fails to augment ET-1 action in the skeletal muscle vascular bed in vivo in humans.American journal of physiology. Endocrinology and metabolism · 2008Trial
- Interplay of fatty acids, insulin and exercise in vascular health.Lipids in health and disease · 2025Review
- Improvement of Vascular Insulin Sensitivity by Ranolazine.International journal of molecular sciences · 2023Article
- Common metabolic features of hypertension and type 2 diabetes.Hypertension research : official journal of the Japanese Society of Hypertension · 2023Review
- New insights into mechanisms of endothelial insulin resistance in type 2 diabetes.American journal of physiology. Heart and circulatory physiology · 2022Review
- Role of the Autonomic Nervous System in the Hemodynamic Response to Hyperinsulinemia-Implications for Obesity and Insulin Resistance.Current diabetes reports · 2022Review
- Diabetes pathogenesis and management: the endothelium comes of age.Journal of molecular cell biology · 2021Article
- Investigation of proteins important for microcirculation using in vivo microdialysis after glucose provocation: a proteomic study.Scientific reports · 2021Observational
- Effects of imatinib on vascular insulin sensitivity and free fatty acid transport in early weight gain.PloS one · 2021Article
- Skeletal muscle microvascular insulin resistance in type 2 diabetes is not improved by eight weeks of regular walking.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- Exercise and metabolic health: beyond skeletal muscle.Diabetologia · 2020Review
- GLP-1 and insulin regulation of skeletal and cardiac muscle microvascular perfusion in type 2 diabetes.Journal of diabetes · 2020Review
- Sympathetically mediated increases in cardiac output, not restraint of peripheral vasodilation, contribute to blood pressure maintenance during hyperinsulinemia.American journal of physiology. Heart and circulatory physiology · 2020Article
- Persistent insulin signaling coupled with restricted PI3K activation causes insulin-induced vasoconstriction.American journal of physiology. Heart and circulatory physiology · 2019Article
- Exercise and Vascular Insulin Sensitivity in the Skeletal Muscle and Brain.Exercise and sport sciences reviews · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo determine the roles of nitric oxide, endothelin-1 and phosphatidylinositol 3-kinase (PI3-kinase) in acute responses of isolated rat skeletal muscle arterioles to insulin.
methodsRat cremaster first order arterioles were separated from surrounding tissue, cannulated in a pressure myograph and responses to insulin (4 microU/ml-3.4 mU/ml) were studied without intraluminal blood or flow.
resultsInsulin alone did not significantly affect arteriolar diameter. Non-selective antagonism of endothelin receptors, with PD-142893, uncovered insulin-induced vasodilatation (25+/-8% from baseline at 3.4 mU/ml), which was abolished by inhibition of NO synthesis with N(G)-nitro-L-arginine (L-NA). Inhibition of NO synthesis alone uncovered insulin-induced vasoconstriction at physiological concentrations (21+/-5% from baseline diameter at 34 microU/ml), which was abolished by PD-142893. The NO donor, S-nitroso-N-acetyl-penicillamine (SNAP) inhibited insulin-induced vasoconstriction during NOS inhibition, even at a concentration that did not elicit vasodilatation itself. Inhibition of PI3-kinase, an intracellular mediator of insulin-induced NO production, with wortmannin, also uncovered insulin-induced vasoconstriction (13+/-3% from baseline at 34 microU/ml) that was abolished by PD-142893.
conclusionsInsulin induces both nitric oxide and endothelin-1 activity in rat cremaster first-order arterioles. This study demonstrates for the first time that vasoconstrictive effects of physiological concentrations of insulin during inhibition of NOS activity are mediated by endothelin and that insulin induces endothelin-1-mediated vasoconstriction in isolated skeletal muscle arterioles during inhibition of PI3-kinase. These findings support the hypothesis of altered microvascular reactivity to insulin in conditions of diminished PI3-kinase activity, a prominent feature of insulin resistance.
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