ArticleNucleic acids research2002
The 3' untranslated region of human vimentin mRNA interacts with protein complexes containing eEF-1gamma and HAX-1.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 84 citations in OpenAlex.
- Function of eEF-1γ in the nucleus in response to insulin in hepatocellular carcinoma cells.Communications biology · 2025Article
- Review
- Molecular functions of HAX1 during disease progress.Virus genes · 2024Review
- Analysis of the effects of importin α1 on the nuclear translocation of IL-1α in HeLa cells.Scientific reports · 2024Article
- The RNA-Binding Landscape of HAX1 Protein Indicates Its Involvement in Translation and Ribosome Assembly.Cells · 2022Article
- The evolution of vimentin and desmin inFrontiers in physiology · 2022Article
- Identification of host factors binding to dengue and Zika virus subgenomic RNA by efficient yeast three-hybrid screens of the human ORFeome.RNA biology · 2021Article
- The interactome of multifunctional HAX1 protein suggests its role in the regulation of energy metabolism, de-aggregation, cytoskeleton organization and RNA-processing.Bioscience reports · 2020Article
- Metallothionein-3 as a multifunctional player in the control of cellular processes and diseases.Molecular brain · 2020Review
- HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility.Molecular biology of the cell · 2019Article
- Abnormal expression of HAX‑1 is associated with cellular proliferation and migration in human hypopharyngeal squamous cell carcinoma.Molecular medicine reports · 2017Article
- Kostmann's Disease and HCLS1-Associated Protein X-1 (HAX1).Journal of clinical immunology · 2017Review
- Targeted Genetic Screen in Amyotrophic Lateral Sclerosis Reveals Novel Genetic Variants with Synergistic Effect on Clinical Phenotype.Frontiers in molecular neuroscience · 2017Article
- eEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts.Journal of experimental & clinical cancer research : CR · 2016Article
- Clinical and biological significance of HAX-1 overexpression in nasopharyngeal carcinoma.Oncotarget · 2016Article
- The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner.BMC molecular biology · 2015Article
- HAX-1: a novel p-body protein.DNA and cell biology · 2015Article
- Protein kinase Darkener of apricot and its substrate EF1γ regulate organelle transport along microtubules.Journal of cell science · 2014Article
- Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis.Nature medicine · 2012Article
- Centrosomal localization of the psoriasis candidate gene product, CCHCR1, supports a role in cytoskeletal organization.PloS one · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Previously, we have shown that the vimentin 3' untranslated region (3'UTR) contains a highly conserved region, which is sufficient for the perinuclear localization of a reporter mRNA. This region was shown to specifically bind protein(s) by band shift analyses. UV-cross-linking studies suggest these proteins are 46- and 35-kDa in mass. Here, we have used this sequence as 'bait' to isolate RNA binding proteins using the yeast three-hybrid method. This technique relies on a functional assay detecting bona fide RNA-protein interaction in vivo. Three cDNA isolates, HAX-1, eEF-1gamma and hRIP, code for proteins of a size consistent with in vitro cross- linking studies. In all cases, recombinant proteins were capable of binding RNA in vitro. Although hRIP is thought to be a general mRNA binding protein, this represents an unreported activity for eEF-1gamma and HAX-1. Moreover, HAX-1 binding appears to be specific to vimentin's 3'UTR. Both in vivo synthesized eEF-1gamma and HAX-1 proteins were 'pulled out' of HeLa whole cell extracts by binding to a RNA affinity column comprised of vimentin's 3'UTR. Moreover, size-fractionation of extracts results in the separation of large complexes containing either eEF-1gamma or HAX-1. Thus, in addition to their known functions, both eEF-1gamma and HAX-1 are RNA binding proteins, which suggests new roles in mRNA translation and/or perinuclear localization.
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