ArticlePflugers Archiv : European journal of physiology2003
Photolithographically defined deposition of attachment factors as a versatile method for patterning the growth of different cell types in culture.
Article in Pflugers Archiv : European journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- Calmodulin binds to the N-terminal domain of the cardiac sodium channel NaChannels (Austin, Tex.) · 2020Article
- Nanovolcano microelectrode arrays: toward long-term on-demand registration of transmembrane action potentials by controlled electroporation.Microsystems & nanoengineering · 2020Article
- The Role of Membrane Capacitance in Cardiac Impulse Conduction: An Optogenetic Study With Non-excitable Cells Coupled to Cardiomyocytes.Frontiers in physiology · 2020Article
- High-speed mechano-active multielectrode array for investigating rapid stretch effects on cardiac tissue.Nature communications · 2019Article
- Electrophysiological Properties and Viability of Neonatal Rat Ventricular Myocyte Cultures with Inducible ChR2 Expression.Scientific reports · 2017Article
- Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: Mechanisms and model systems.Journal of molecular and cellular cardiology · 2016Review
- Myofibroblasts Electrotonically Coupled to Cardiomyocytes Alter Conduction: Insights at the Cellular Level from a DetailedFrontiers in physiology · 2016Article
- Isolation and cryopreservation of neonatal rat cardiomyocytes.Journal of visualized experiments : JoVE · 2015Article
- Optical recording of calcium currents during impulse conduction in cardiac tissue.Neurophotonics · 2015Article
- Functional interaction between charged nanoparticles and cardiac tissue: a new paradigm for cardiac arrhythmia?Nanomedicine (London, England) · 2013Article
- Fibroblast KATP currents modulate myocyte electrophysiology in infarcted hearts.American journal of physiology. Heart and circulatory physiology · 2013Article
- Biomimetic polymer brushes containing tethered acetylcholine analogs for protein and hippocampal neuronal cell patterning.Biomacromolecules · 2013Article
- Spatial gradients in action potential duration created by regional magnetofection of hERG are a substrate for wavebreak and turbulent propagation in cardiomyocyte monolayers.The Journal of physiology · 2012Article
- Cross talk between cardiac myocytes and fibroblasts: from multiscale investigative approaches to mechanisms and functional consequences.American journal of physiology. Heart and circulatory physiology · 2012Review
- Ionic mechanism of shock-induced arrhythmias: role of intracellular calcium.Heart rhythm · 2012Article
- A protective antiarrhythmic role of ursodeoxycholic acid in an in vitro rat model of the cholestatic fetal heart.Hepatology (Baltimore, Md.) · 2011Article
- On-chip constructive cell-network study (I): contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect.Journal of nanobiotechnology · 2011Article
- Structural heterogeneity promotes triggered activity, reflection and arrhythmogenesis in cardiomyocyte monolayers.The Journal of physiology · 2011Article
- Gelatin-based laser direct-write technique for the precise spatial patterning of cells.Tissue engineering. Part C, Methods · 2011Article
- Enhanced fibroblast-myocyte interactions in response to cardiac injury.Circulation research · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatially defined growth of cells in culture is a useful model for studies ranging from the characterization of cellular motility to the analysis of network behaviour in structurally defined ensembles of excitable cells. Current methodological approaches for obtaining patterned growth include sophisticated modifications of surface chemistry, stamping techniques and microfluidics. The implementation of most of these techniques requires the availability of highly specialized apparatus and some of the methods are specific for certain cell types and/or substrate materials. The goal of the present study was to develop a cell-patterning technique that can be implemented by any laboratory working with cell culture and that is highly adaptable in terms of cell types and substrate materials. The method is based on a photolithographic process that permits the patterned deposition of attachment factors of choice on surfaces previously coated with agar with a spatial resolution (maximal deviation from a straight line) of +/-3 micro m. Because agar efficiently prevents cell adhesion, patterned growth obtained with this technique displays virtually no off-pattern cell attachment. The method permitted the patterning of cardiomyocytes, fibroblasts and HeLa cells on either glass substrates or polymer-coated materials with a spatial resolution of a few micrometers.
Indexed as
Identifiers
12690471What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.