ArticleMolecular genetics and genomics : MGG2003
Electroporation of isolated higher-plant mitochondria: transcripts of an introduced cox2 gene, but not an atp6 gene, are edited in organello.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Conservation of the moss RNA editing factor PPR78 despite the loss of its known cytidine-to-uridine editing sites is explained by a hidden extra target.The Plant cell · 2024Article
- The P-type pentatricopeptide repeat protein DWEORG1 is a non-previously reported rPPR protein of Arabidopsis mitochondria.Scientific reports · 2022Article
- Article
- RNA Recognition Motif-Containing Protein ORRM4 Broadly Affects Mitochondrial RNA Editing and Impacts Plant Development and Flowering.Plant physiology · 2016Article
- Two RNA recognition motif-containing proteins are plant mitochondrial editing factors.Nucleic acids research · 2015Article
- Comprehensive high-resolution analysis of the role of an Arabidopsis gene family in RNA editing.PLoS genetics · 2013Article
- Deletions in cox2 mRNA result in loss of splicing and RNA editing and gain of novel RNA editing sites.PloS one · 2013Article
- RNA processing in plant mitochondria is independent of transcription.Plant molecular biology · 2009Article
- The BEM46-like protein appears to be essential for hyphal development upon ascospore germination in Neurospora crassa and is targeted to the endoplasmic reticulum.Current genetics · 2009Article
- Mitochondrial mRNA polymorphisms in different Arabidopsis accessions.Plant physiology · 2008Article
- Article
- Plastid mRNAs are neither spliced nor edited in maize and cauliflower mitochondrial in organello systems.RNA (New York, N.Y.) · 2007Article
- An in vitro RNA editing system from cauliflower mitochondria: editing site recognition parameters can vary in different plant species.RNA (New York, N.Y.) · 2005Article
- Mitochondrial electroporation and in organello RNA editing of chimeric atp6 transcripts.Molecular genetics and genomics : MGG · 2005Article
- Gene expression studies in isolated mitochondria: Solanum tuberosum rps10 is recognized by cognate potato but not by the transcription, splicing and editing machinery of wheat mitochondria.Nucleic acids research · 2005Article
- Complex cis-elements determine an RNA editing site in pea mitochondria.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To facilitate the analysis of RNA processing in plant mitochondria, a method was established for introducing foreign DNA into mitochondria isolated from maize and sorghum. This method permits the uptake of DNA of up to 11 kb into the mitochondrial matrix. In vitro incubation of maize mitochondria in a specific buffer system was found to permit splicing and editing of newly synthesized RNAs for a period of at least 7 h. This was shown both for transcripts of endogenous mitochondrial genes (atp6, cox2) and for transcripts derived from an introduced Arabidopsis thaliana cox2 gene. In contrast, when a Sorghum bicolor atp6 gene was introduced into isolated maize mitochondria, the gene was transcribed, but the RNA was not edited, although all the editing sites in maize and sorghum atp6 RNA are identical. This may indicate the presence of transcript-specific cis -acting regions in the up- or downstream untranslated sequences of the mRNA. The system described here should allow further dissection of the mechanism of RNA editing in plant mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.