ArticleMolecular and cellular biology2003
Human mediator enhances activator-facilitated recruitment of RNA polymerase II and promoter recognition by TATA-binding protein (TBP) independently of TBP-associated factors.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- BRD4: From molecular understanding to therapeutics development.Molecular cell · 2026Review
- ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss.Molecular cell · 2024Article
- The Mediator complex as a master regulator of transcription by RNA polymerase II.Nature reviews. Molecular cell biology · 2022Review
- Article
- CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.Cell death and differentiation · 2021Article
- MedProDB: A database of Mediator proteins.Computational and structural biotechnology journal · 2021Article
- Essential role of MED1 in the transcriptional regulation of ER-dependent oncogenic miRNAs in breast cancer.Scientific reports · 2018Article
- BRD3 and BRD4 BET Bromodomain Proteins Differentially Regulate Skeletal Myogenesis.Scientific reports · 2017Article
- Article
- Epigenetic modification of histone 3 lysine 27: mediator subunit MED25 is required for the dissociation of polycomb repressive complex 2 from the promoter of cytochrome P450 2C9.The Journal of biological chemistry · 2015Article
- A functional portrait of Med7 and the mediator complex in Candida albicans.PLoS genetics · 2014Article
- Brd4 and JMJD6-associated anti-pause enhancers in regulation of transcriptional pause release.Cell · 2013Article
- Article
- The Mediator complex and transcription elongation.Biochimica et biophysica acta · 2013Review
- Cyclin-dependent kinase 8 positively cooperates with Mediator to promote thyroid hormone receptor-dependent transcriptional activation.Molecular and cellular biology · 2010Article
- Single cell analysis of transcriptional activation dynamics.PloS one · 2010Article
- RNA polymerase II C-terminal heptarepeat domain Ser-7 phosphorylation is established in a mediator-dependent fashion.The Journal of biological chemistry · 2010Article
- The basal initiation machinery: beyond the general transcription factors.Current opinion in cell biology · 2009Review
- Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding.The EMBO journal · 2009Article
- Chromatin adaptor Brd4 modulates E2 transcription activity and protein stability.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Mediator is a general cofactor implicated in the functions of many transcriptional activators. Although Mediator with different protein compositions has been isolated, it remains unclear how Mediator facilitates activator-dependent transcription, independent of its general stimulation of basal transcription. To define the mechanisms of Mediator function, we isolated two forms of human Mediator complexes (Mediator-P.5 and Mediator-P.85) and demonstrated that Mediator-P.5 clearly functions by enhancing activator-mediated recruitment of RNA polymerase II (pol II), whereas Mediator-P.85 works mainly by stimulating overall basal transcription. The coactivator function of Mediator-P.5 was not impaired when TATA-binding protein (TBP) was used in place of TFIID, but it was abolished when another general cofactor, PC4, was omitted from the reaction or when Mediator-P.5 was added after pol II entry into the preinitiation complex. Moreover, Mediator- P.5 is able to enhance TBP binding to the TATA box in an activator-dependent manner. Our data provides biochemical evidence that Mediator functions by facilitating activator-mediated recruitment of pol II and also promoter recognition by TBP, both of which can occur in the absence of TBP-associated factors in TFIID.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.