ArticleThe Biochemical journal1992
Carbachol stimulation of phospholipase A2 and insulin secretion in pancreatic islets.
Article in The Biochemical journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 55 citations in OpenAlex.
- Designing a bioelectronic treatment for Type 1 diabetes: targeted parasympathetic modulation of insulin secretion.Bioelectronics in medicine · 2020Review
- Assessment of re-aggregated human pancreatic islets for secondary drug screening.British journal of pharmacology · 2014Article
- STIM and Orai proteins and the non-capacitative ARC channels.Frontiers in bioscience (Landmark edition) · 2012Review
- Modulation of the pancreatic islet beta-cell-delayed rectifier potassium channel Kv2.1 by the polyunsaturated fatty acid arachidonate.The Journal of biological chemistry · 2007Article
- Group VIA phospholipase A2 forms a signaling complex with the calcium/calmodulin-dependent protein kinase IIbeta expressed in pancreatic islet beta-cells.The Journal of biological chemistry · 2005Article
- Inhibition of Ca2+-independent phospholipase A2 results in insufficient insulin secretion and impaired glucose tolerance.Molecular endocrinology (Baltimore, Md.) · 2005Article
- Phospholipase A2 and its potential regulation of islet function.International journal of pancreatology : official journal of the International Association of Pancreatology · 2000Review
- Dual pathways for carbamylcholine-stimulated arachidonic acid release in rat pancreatic acini.Endocrine · 1996Article
- Muscarinic stimulation exerts both stimulatory and inhibitory effects on the concentration of cytoplasmic Ca2+ in the electrically excitable pancreatic B-cell.The Biochemical journal · 1995Article
- The pathogenesis of NIDDM: the role of the pancreatic beta cell.Diabetologia · 1995Review
- Arachidonic acid and diacylglycerol release associated with inhibition of myosin light chain dephosphorylation in rabbit smooth muscle.The Journal of physiology · 1995Article
- Regulation of phosphatidylinositol kinases by arachidonic acid in rat submandibular gland cells.Pflugers Archiv : European journal of physiology · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Arachidonic acid has been implicated as a second messenger in insulin secretion by islets of Langerhans. D-Glucose, the major physiological stimulus, increases unesterified arachidonate accumulation in islets. We now show, for the first time, that the muscarinic agonist carbachol, at concentrations which stimulate insulin secretion, causes a rapid and nearly 3-fold increase in arachidonic acid accumulation in islets. The combination of glucose and carbachol has an additive effect on unesterified arachidonate release. There is a large component of secretagogue-induced arachidonate accumulation that is independent of extracellular Ca2+. Carbachol stimulation of arachidonic acid release is mediated by activation of phospholipase A2, as demonstrated by early increases in endogenous lysophosphatidylcholine. In addition to phospholipase A2 activation, carbachol-induced arachidonic acid accumulation also appears to involve diacylglycerol hydrolysis, since the diacylglycerol lipase inhibitor RG80267 partly inhibited arachidonic acid accumulation. In contrast, glucose-induced arachidonic acid accumulation appears to reflect diacylglycerol hydrolysis entirely. Our observations indicate that phospholipase A2 has an important role in muscarinic-induced insulin secretion.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.