ArticleAnalytical biochemistry1992
A mass spectrometric method for measuring glycerol levels and enrichments in plasma using 13C and 2H stable isotopic tracers.
Article in Analytical biochemistry, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02045290 (Metformin Therapy for Overweight Adolescents With Type 1 Diabetes), which is not on this map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Metformin Therapy for Overweight Adolescents With Type 1 Diabetes (T1D)--Insulin Clamp Ancillary Study for Assessment of Insulin Resistance
Who cites it
19 citing papers in PubMed.
- Metformin Improves Peripheral Insulin Sensitivity in Youth With Type 1 Diabetes.The Journal of clinical endocrinology and metabolism · 2019Trial
- Acute modulation of adipose tissue lipolysis by intravenous estrogens.Obesity (Silver Spring, Md.) · 2006 · on this mapTrial
- Insulin Sensitivity and Insulin Secretion in Adults With Friedreich's Ataxia: The Role of Skeletal Muscle.The Journal of clinical endocrinology and metabolism · 2025Observational
- Good agreement between hyperinsulinemic-euglycemic clamp and 2 hours oral minimal model assessed insulin sensitivity in adolescents.Pediatric diabetes · 2020Article
- Muscle Insulin Resistance in Youth with Obesity and Normoglycemia is Associated with Altered Fat Metabolism.Obesity (Silver Spring, Md.) · 2019Article
- Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization.Analytical biochemistry · 2019Article
- Nonalcoholic fatty liver disease in obese adolescent females is associated with multi-tissue insulin resistance and visceral adiposity markers.Metabolism open · 2019Article
- Youth with type 2 diabetes have hepatic, peripheral, and adipose insulin resistance.American journal of physiology. Endocrinology and metabolism · 2019Article
- Youth With Type 1 Diabetes Have Adipose, Hepatic, and Peripheral Insulin Resistance.The Journal of clinical endocrinology and metabolism · 2018Article
- Oral Glucose Tolerance Test Glucose Peak Time Is Most Predictive of Prediabetes and Hepatic Steatosis in Obese Girls.Journal of the Endocrine Society · 2018Article
- Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads.Journal of visualized experiments : JoVE · 2017Article
- Insulin Resistance, Hyperinsulinemia, and Mitochondria Dysfunction in Nonobese Girls With Polycystic Ovarian Syndrome.Journal of the Endocrine Society · 2017Article
- Effects of CETP inhibition with anacetrapib on metabolism of VLDL-TG and plasma apolipoproteins C-II, C-III, and E.Journal of lipid research · 2017Article
- Hepatic Steatosis is Common in Adolescents with Obesity and PCOS and Relates to De Novo Lipogenesis but not Insulin Resistance.Obesity (Silver Spring, Md.) · 2016Article
- Modeling changes in glucose and glycerol rates of appearance when true basal rates of appearance cannot be readily determined.American journal of physiology. Endocrinology and metabolism · 2016Article
- Subcutaneous adipose tissue insulin resistance is associated with visceral adiposity in postmenopausal women.Obesity (Silver Spring, Md.) · 2014Article
- A novel index of whole body antilipolytic insulin action.Obesity (Silver Spring, Md.) · 2013Article
- Impaired muscle protein anabolic response to insulin and amino acids in heart failure patients: relationship with markers of immune activation.Clinical science (London, England : 1979) · 2010Article
- Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment.Journal of the American Society for Mass Spectrometry · 2005Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The stable isotope tracer [1,1,2,3,3,-2H5]glycerol has been commonly used as a tracer to measure glycerol kinetics and lipolysis in vivo. The method for measuring samples using the trimethylsilyl derivative and electron impact gas chromatograph-mass spectrometry retains only three of the five deuteriums, resulting in the possibility of incorrectly identifying the whole glycerol tracer molecule. This reports preparation of glycerol as the heptafluorobutyrl derivative and measurement by negative ion chemical ionization gas chromatography-mass spectrometry to produce a derivative with an intense molecular ion that retains all five deuterium labels. Thus the heptafluorobutyrl derivative analyzed by negative ion mass spectrometry overcomes the problems associated with fragmentation and loss of the isotopic label. Glycerol concentration using a labeled internal standard can be determined in plasma with a precision of 3%. Nanomole amounts of glycerol can be analyzed for 13C or 2H enrichments with a precision of +/- 0.14 mol% excess isotope. This simple, sensitive method for measuring glycerol levels and stable isotopic enrichment in plasma uses a simple extraction procedure and requires a minimal volume of plasma (less than 300 microliters).
Indexed as
Identifiers
1443555What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.