Trial reportThe Journal of physiology2003
Intramyocellular lipids form an important substrate source during moderate intensity exercise in endurance-trained males in a fasted state.
Trial report in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 3 syntheses or guidelines pooled it, 223 citations in OpenAlex.
- Genetics of Exercise and Diet-Induced Fat Loss Efficiency: A Systematic Review.Journal of sports science & medicine · 2024Pooled it
- The Importance of Lipidomic Approach for Mapping and Exploring the Molecular Networks Underlying Physical Exercise: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- No Influence of Overweight/Obesity on Exercise Lipid Oxidation: A Systematic Review.International journal of molecular sciences · 2020Pooled it
- New Zealand blackcurrant extract augments muscle glycogen storage at rest and enhances intramuscular triglyceride degradation during prolonged exercise.European journal of applied physiology · 2026Trial
- Substituting Carbohydrate at Lunch for Added Protein Increases Fat Oxidation During Subsequent Exercise in Healthy Males.The Journal of clinical endocrinology and metabolism · 2025Trial
- Effect of electromyostimulation on intramyocellular lipids of the vastus lateralis in older adults: a randomized controlled trial.BMC musculoskeletal disorders · 2021Trial
- Taurine Supplementation Increases Post-Exercise Lipid Oxidation at Moderate Intensity in Fasted Healthy Males.Nutrients · 2020Trial
- Effect of ubiquinol supplementation on biochemical and oxidative stress indexes after intense exercise in young athletes.Redox report : communications in free radical research · 2018Trial
- Intramyocellular triacylglycerol accumulation across weight loss strategies; Sub-study of the CENTRAL trial.PloS one · 2017Trial
- The effect of aerobic exercise on intrahepatocellular and intramyocellular lipids in healthy subjects.PloS one · 2013Trial
- Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes.American journal of physiology. Endocrinology and metabolism · 2012Trial
- Intramyocellular lipid stores increase markedly in athletes after 1.5 days lipid supplementation and are utilized during exercise in proportion to their content.European journal of applied physiology · 2006Trial
- Inhibition of adipose tissue lipolysis increases intramuscular lipid use in type 2 diabetic patients.Diabetologia · 2005Trial
- Exercise in the fasted state facilitates fibre type-specific intramyocellular lipid breakdown and stimulates glycogen resynthesis in humans.The Journal of physiology · 2005Trial
- Exercise and Fat: A Primer.Sports medicine - open · 2026Review
- What Do We Know of Human Fuel Use during Aerobic Exercise, and How Do We Know It?Physiology (Bethesda, Md.) · 2026Review
- The Role of Exercise in Fat Oxidation and Its Impact on Adolescent Metabolism.Advances in experimental medicine and biology · 2026Review
- Characterizing 24-Hour Skeletal Muscle Gene Expression Alongside Metabolic and Endocrine Responses Under Diurnal Conditions.The Journal of clinical endocrinology and metabolism · 2025Article
- Quantification of nutrient fluxes during acute exercise in mice.Cell metabolism · 2024Article
- Exercise-induced BDNF promotes PPARδ-dependent reprogramming of lipid metabolism in skeletal muscle during exercise recovery.Science signaling · 2024Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Both stable isotope methodology and fluorescence microscopy were applied to define the use of intramuscular triglyceride (IMTG) stores as a substrate source during exercise on a whole-body as well as on a fibre type-specific intramyocellular level in trained male cyclists. Following an overnight fast, eight subjects were studied at rest, during 120 min of moderate intensity exercise (60 % maximal oxygen uptake capacity (VO2,max)) and 120 min of post-exercise recovery. Continuous infusions of [U-13C]palmitate and [6,6-2H2]glucose were administered at rest and during subsequent exercise to quantify whole-body plasma free fatty acid (FFA) and glucose oxidation rates and the contribution of other fat sources (sum of muscle- plus lipoprotein-derived TG) and muscle glycogen to total energy expenditure. Fibre type-specific intramyocellular lipid content was determined in muscle biopsy samples collected before, immediately after and 2 h after exercise. At rest, fat oxidation provided 66 +/- 5 % of total energy expenditure, with FFA and other fat sources contributing 48 +/- 6 and 17 +/- 3 %, respectively. FFA oxidation rates increased during exercise, and correlated well with the change in plasma FFA concentrations. Both the use of other fat sources and muscle glycogen declined with the duration of exercise, whereas plasma glucose production and utilisation increased (P < 0.001). On average, FFA, other fat sources, plasma glucose and muscle glycogen contributed 28 +/- 3, 15 +/- 2, 12 +/- 1 and 45 +/- 4 % to total energy expenditure during exercise, respectively. Fluorescence microscopy revealed a 62 +/- 7 % net decline in muscle lipid content following exercise in the type I fibres only, with no subsequent change during recovery. We conclude that IMTG stores form an important substrate source during moderate intensity exercise in endurance-trained male athletes following an overnight fast, with the oxidation rate of muscle- plus lipoprotein-derived TG being decreased with the duration of exercise.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.