ArticleMolecular biology of the cell2004
Roles of phosphoinositides and of Spo14p (phospholipase D)-generated phosphatidic acid during yeast sporulation.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 60 citations in OpenAlex.
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- Roles for the canonical polarity machinery in the de novo establishment of polarity in budding yeast spores.Molecular biology of the cell · 2025Article
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- Recruitment of the lipid kinase Mss4 to the meiotic spindle pole promotes prospore membrane formation inMolecular biology of the cell · 2023Article
- Article
- Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension.PLoS genetics · 2021Article
- Tubular ER Associates With Diacylglycerol-Rich Structures During Lipid Droplet Consumption.Frontiers in cell and developmental biology · 2020Article
- Developmental regulation of an organelle tether coordinates mitochondrial remodeling in meiosis.The Journal of cell biology · 2019Article
- Target Identification and Mechanism of Action of Picolinamide and Benzamide Chemotypes with Antifungal Properties.Cell chemical biology · 2018Article
- Lipid droplets are central organelles for meiosis II progression during yeast sporulation.Molecular biology of the cell · 2017Article
- Assembly, molecular organization, and membrane-binding properties of development-specific septins.The Journal of cell biology · 2016Article
- SPO71 encodes a developmental stage-specific partner for Vps13 in Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- SPO71 mediates prospore membrane size and maturation in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- VPS13 regulates membrane morphogenesis during sporulation in Saccharomyces cerevisiae.Journal of cell science · 2012Article
- Sec14 like PITPs couple lipid metabolism with phosphoinositide synthesis to regulate Golgi functionality.Sub-cellular biochemistry · 2012Review
- DGK1-encoded diacylglycerol kinase activity is required for phospholipid synthesis during growth resumption from stationary phase in Saccharomyces cerevisiae.The Journal of biological chemistry · 2011Article
- Multiple functions as lipase, steryl ester hydrolase, phospholipase, and acyltransferase of Tgl4p from the yeast Saccharomyces cerevisiae.The Journal of biological chemistry · 2010Article
- The Sec14 superfamily and mechanisms for crosstalk between lipid metabolism and lipid signaling.Trends in biochemical sciences · 2010Review
- Janus-faced enzymes yeast Tgl3p and Tgl5p catalyze lipase and acyltransferase reactions.Molecular biology of the cell · 2010Article
- Phospholipase D in the Golgi apparatus.Biochimica et biophysica acta · 2009Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
During yeast sporulation, internal membrane synthesis ensures that each haploid nucleus is packaged into a spore. Prospore membrane formation requires Spo14p, a phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]-stimulated phospholipase D (PLD), which hydrolyzes phosphatidylcholine (PtdCho) to phosphatidic acid (PtdOH) and choline. We found that both meiosis and spore formation also require the phosphatidylinositol (PtdIns)/PtdCho transport protein Sec14p. Specific ablation of the PtdIns transport activity of Sec14p was sufficient to impair spore formation but not meiosis. Overexpression of Pik1p, a PtdIns 4-kinase, suppressed the sec14-1 meiosis and spore formation defects; conversely, pik1-ts diploids failed to undergo meiosis and spore formation. The PtdIns(4)P 5-kinase, Mss4p, also is essential for spore formation. Use of phosphoinositide-specific GFP-PH domain reporters confirmed that PtdIns(4,5)P2 is enriched in prospore membranes. sec14, pik1, and mss4 mutants displayed decreased Spo14p PLD activity, whereas absence of Spo14p did not affect phosphoinositide levels in vivo, suggesting that formation of PtdIns(4,5)P2 is important for Spo14p activity. Spo14p-generated PtdOH appears to have an essential role in sporulation, because treatment of cells with 1-butanol, which supports Spo14p-catalyzed PtdCho breakdown but leads to production of Cho and Ptd-butanol, blocks spore formation at concentrations where the inert isomer, 2-butanol, has little effect. Thus, rather than a role for PtdOH in stimulating PtdIns(4,5)P2 formation, our findings indicate that during sporulation, Spo14p-mediated PtdOH production functions downstream of Sec14p-, Pik1p-, and Mss4p-dependent PtdIns(4,5)P2 synthesis.
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