ArticleEukaryotic cell2003
A negative regulatory element controls mRNA abundance of the Leishmania mexicana Paraflagellar rod gene PFR2.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Decoding stoichiometric protein synthesis inBiophysical reports · 2023Article
- Quantitative single-cell analysis of Leishmania major amastigote differentiation demonstrates variably extended expression of the lipophosphoglycan (LPG) virulence factor in different host cell types.PLoS neglected tropical diseases · 2022Article
- Finding Correlations Between mRNA and Protein Levels inFrontiers in cellular and infection microbiology · 2022Review
- Catalase impairsVirulence · 2021Article
- Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation.Scientific reports · 2019Article
- Basal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellum.Nature communications · 2015Article
- Comparative Life Cycle Transcriptomics Revises Leishmania mexicana Genome Annotation and Links a Chromosome Duplication with Parasitism of Vertebrates.PLoS pathogens · 2015Article
- Species-specific antimonial sensitivity in Leishmania is driven by post-transcriptional regulation of AQP1.PLoS neglected tropical diseases · 2015Article
- A short RNA stem-loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes.Nucleic acids research · 2014Article
- Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization.Parasites & vectors · 2011Article
- Article
- Article
- The paraflagellar rod of kinetoplastid parasites: from structure to components and function.International journal for parasitology · 2010Review
- Functional genomic characterization of mRNAs associated with TcPUF6, a pumilio-like protein from Trypanosoma cruzi.The Journal of biological chemistry · 2008Article
- Coordinate regulation of a family of promastigote-enriched mRNAs by the 3'UTR PRE element in Leishmania mexicana.Molecular and biochemical parasitology · 2008Article
- Members of a large retroposon family are determinants of post-transcriptional gene expression in Leishmania.PLoS pathogens · 2007Article
- Interacting protein kinases involved in the regulation of flagellar length.Molecular biology of the cell · 2006Article
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Authors and funding
4 authors.
Funding
Abstract
The Leishmania mexicana PFR2 locus encodes a component of the paraflagellar rod (PFR), a flagellar structure found only in the insect stage of the life cycle. PFR2 mRNA levels are 10-fold lower in the mammalian stage than in the insect stage. Nuclear run-on experiments indicate that the change in PFR2 mRNA abundance is achieved posttranscriptionally. Deletion and block substitution analysis of the entire 1,400-nucleotide 3' untranslated region (UTR) of PFR2C led to the identification of a regulatory element contained within 10 nucleotides of the 3' UTR, termed the PFR regulatory element (PRE), that is necessary for the 10-fold regulation of PFR2 mRNA levels. Comparison of the half-lives of PFR2 transcripts, identical except for the presence or absence of the PRE, revealed that the PRE acts by destabilizing the PFR2 mRNA in amastigotes. The PRE was inserted into a construct which directs the constitutive expression of a chimeric PFR2 transcript. Insertion of the PRE resulted in regulated expression of this transcript, demonstrating that the regulatory element is sufficient for promastigote-specific expression. Since the PRE is present in the 3' UTR of all L. mexicana PFR genes examined so far, we propose that it serves a means of coordinating expression of PFR genes.
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