ArticlePflugers Archiv : European journal of physiology2003
Constitutive nitric oxide synthases in rat pancreatic islets: direct imaging of glucose-induced nitric oxide production in beta-cells.
Article in Pflugers Archiv : European journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Ryanodine Receptors in Islet Cell Function: Calcium Signaling, Hormone Secretion, and Diabetes.Cells · 2025Review
- Article
- Human Nitric Oxide Synthase-Its Functions, Polymorphisms, and Inhibitors in the Context of Inflammation, Diabetes and Cardiovascular Diseases.International journal of molecular sciences · 2020Review
- Insulin secretion: The nitric oxide controversy.EXCLI journal · 2020Review
- Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.The Journal of biological chemistry · 2011Article
- Oxidative stress and beta-cell dysfunction.Pflugers Archiv : European journal of physiology · 2010Review
- Role of nitric oxide on purinergic signalling in the cochlea.Purinergic signalling · 2010Article
- Microfluidic device for multimodal characterization of pancreatic islets.Lab on a chip · 2009Article
- Dual effect of nitric oxide on ATP-sensitive K+ channels in rat pancreatic beta cells.Pflugers Archiv : European journal of physiology · 2008Article
- Free radicals and the pancreatic acinar cells: role in physiology and pathology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2005Review
- Nutrient-stimulated insulin secretion in mouse islets is critically dependent on intracellular pH.BMC endocrine disorders · 2004Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of nitric oxide (NO) by constitutive nitric oxide synthase (NOS) was investigated in isolated rat pancreatic islets and dispersed beta-cells. Double-immunocytochemical analyses with a confocal microscope demonstrated the presence of NOS1 in alpha-, beta-, delta-, and PP-cells and that of NOS3 in beta-, delta-, and PP-cells, but not alpha-cells, in the isolated rat islets. Image analyses with the NO-reactive fluorescence dye DAF-2 clearly showed that an elevation in glucose concentrations from 0 to 11.1 mM increased intracellular NO in most cells of the isolated islets. The glucose-induced elevation of intracellular NO in the islet cells was abolished in the presence of the Ca2+ channel blocker nicardipine and after treatment with the NOS inhibitor NG-nitro-L-arginine. Similarly, the ATP-sensitive K+ channel blocker tolbutamide, which elevates intracellular Ca2+ concentrations, increased DAF-2 fluorescence in most cells of the isolated islets. In isolated beta-cells, 11.1 mM glucose increased DAF-2 fluorescence, which was suppressed by NG-nitro-L-arginine and by reducing the glucose concentration to 0 mM. DAF-2 fluorescence in beta-cells was also increased by 50 mM K+, which was suppressed by NG-nitro-L-arginine. These results suggest that NOS1 and NOS3 are present in rat pancreatic beta-cells, and that glucose produces NO by Ca(2+)-dependent activation of the constitutive NOS isoforms.
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