ArticleThe EMBO journal1992
CD45 tyrosine phosphatase-activated p59fyn couples the T cell antigen receptor to pathways of diacylglycerol production, protein kinase C activation and calcium influx.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 175 citations in OpenAlex.
- Regulation of TCR signalling by tyrosine phosphatases: from immune homeostasis to autoimmunity.Immunology · 2012Pooled it
- The protein tyrosine phosphatase CD45 promotes PMN transepithelial migration, antimicrobial function, and colonic mucosal repair.JCI insight · 2026Article
- CD45 as a multifunctional target in immune regulation and therapeutic delivery.Journal of translational medicine · 2026Review
- Effect of MHC Linked 7-Gene Signature on Delayed Hepatocellular Carcinoma Recurrence.Journal of personalized medicine · 2021Article
- Modulation of TCR Signaling by Tyrosine Phosphatases: From Autoimmunity to Immunotherapy.Frontiers in cell and developmental biology · 2020Review
- The Fyn-ADAP Axis: Cytotoxicity Versus Cytokine Production in Killer Cells.Frontiers in immunology · 2015Review
- Article
- The phospholipase C isozymes and their regulation.Sub-cellular biochemistry · 2012Review
- Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148.The Journal of biological chemistry · 2011Article
- Selective regulation of TCR signaling pathways by the CD45 protein tyrosine phosphatase during thymocyte development.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Positive and negative regulation of T-cell activation through kinases and phosphatases.The Biochemical journal · 2003Review
- Integrin-mediated tyrosine phosphorylation of Shc in T cells is regulated by protein kinase C-dependent phosphorylations of Lck.Molecular biology of the cell · 2003Article
- Signal-transduction defects in T cells.Clinical reviews in allergy & immunology · 2001Review
- Development of T-leukaemias in CD45 tyrosine phosphatase-deficient mutant lck mice.The EMBO journal · 2000Article
- Expression of the p56(Lck) Y505F mutation in CD45-deficient mice rescues thymocyte development.Molecular and cellular biology · 1999Article
- Molecular characterization of U937-dependent T-cell co-stimulation.Immunology · 1999Article
- The phosphotyrosine phosphatase SHP-2 participates in a multimeric signaling complex and regulates T cell receptor (TCR) coupling to the Ras/mitogen-activated protein kinase (MAPK) pathway in Jurkat T cells.The Journal of experimental medicine · 1998Article
- CD45 and RPTPalpha display different protein tyrosine phosphatase activities in T lymphocytes.The Biochemical journal · 1997Article
- Rescuing impairment of long-term potentiation in fyn-deficient mice by introducing Fyn transgene.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- The Syk protein tyrosine kinase can function independently of CD45 or Lck in T cell antigen receptor signaling.The EMBO journal · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of the CD45 phosphotyrosine phosphatase in coupling the T cell antigen receptor complex (TCR) to intracellular signals was investigated. CD45- HPB-ALL T cells were transfected with cDNA encoding the CD45RA+B+C- isoform. The tyrosine kinase activity of p59fyn was found to be 65% less in CD45- cells than in CD45+ cells, whereas p56lck kinase activity was comparable in both sub-clones. In CD45- cells the TCR was uncoupled from protein tyrosine phosphorylation, phospholipase C gamma 1 regulation, inositol phosphate production, calcium signals, diacylglycerol production and protein kinase C activation. Restoration of TCR coupling to all these pathways correlated with the increased p59fyn activity observed in CD45-transfected cells. Co-aggregation of CD4- or CD8-p56lck kinase with the TCR in CD45- cells restored TCR-induced protein tyrosine phosphorylation, phospholipase C gamma 1 regulation and calcium signals. Receptor-mediated calcium signals were largely due (60-90%) to Ca2+ influx, and only a minor component (10-40%) was caused by Ca2+ release from intracellular stores. Maximal CD3-mediated Ca2+ influx occurred at CD3 mAb concentrations at which inositol phosphate production was non-detectable. These results indicate that CD45-regulated p59fyn plays a critical role in coupling the TCR to specific intracellular signalling pathways and that CD4- or CD8-p56lck can only restore signal transduction coupling in CD45- cells when brought into close association with the TCR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.