ArticleMolecular and cellular biology2004
Transcription Factors Pax6 and AP-2alpha Interact To Coordinate Corneal Epithelial Repair by Controlling Expression of Matrix Metalloproteinase Gelatinase B.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 93 citations in OpenAlex.
- Sexual Dimorphism Is Associated With Corneal Nerve and Epithelial Alterations and Tear-Film Defects in Pax6 Haploinsufficiency Mouse Model.Investigative ophthalmology & visual science · 2026Article
- Comprehensive 3D epigenomic maps define limbal stem/progenitor cell function and identity.Nature communications · 2022Article
- The genetic architecture of aniridia and Gillespie syndrome.Human genetics · 2019Review
- Embryonic cell migratory capacity is impaired upon exposure to glucose in vivo and in vitro.Birth defects research · 2019Article
- PAX6 Promoter Methylation Correlates with MDA-MB-231 Cell Migration, and Expression of MMP2 and MMP9Asian Pacific journal of cancer prevention : APJCP · 2018Article
- Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia.Molecular therapy. Nucleic acids · 2017Article
- Analysis of compound heterozygotes reveals that the mouse floxed Pax6 (tm1Ued) allele produces abnormal eye phenotypes.Transgenic research · 2016Article
- AP-2β Is a Downstream Effector of PITX2 Required to Specify Endothelium and Establish Angiogenic Privilege During Corneal Development.Investigative ophthalmology & visual science · 2016Article
- Contact-mediated control of radial migration of corneal epithelial cells.Molecular vision · 2016Article
- Inflammatory Biomarkers Profile as Microenvironmental Expression in Keratoconus.Disease markers · 2016Review
- Spatiotemporally Regulated Ablation of Klf4 in Adult Mouse Corneal Epithelial Cells Results in Altered Epithelial Cell Identity and Disrupted Homeostasis.Investigative ophthalmology & visual science · 2015Article
- Whole exome sequence analysis of Peters anomaly.Human genetics · 2014Article
- AP-2α is required after lens vesicle formation to maintain lens integrity.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Molecular basis for MMP9 induction and disruption of epithelial cell-cell contacts by galectin-3.Journal of cell science · 2014Article
- Matrix metalloproteinase (MMP) 9 transcription in mouse brain induced by fear learning.The Journal of biological chemistry · 2013Article
- Functional dissection of the paired domain of Pax6 reveals molecular mechanisms of coordinating neurogenesis and proliferation.Development (Cambridge, England) · 2013Article
- Pax6 downregulation mediates abnormal lineage commitment of the ocular surface epithelium in aqueous-deficient dry eye disease.PloS one · 2013Article
- Ocular surface development and gene expression.Journal of ophthalmology · 2013Article
- Stem cells and corneal epithelial maintenance: insights from the mouse and other animal models.Results and problems in cell differentiation · 2012Review
- Critical role of Klf5 in regulating gene expression during post-eyelid opening maturation of mouse corneas.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
Abstract
Pax6 is a paired box containing transcription factor that resides at the top of a genetic hierarchy controlling eye development. It continues to be expressed in tissues of the adult eye, but its role in this capacity is unclear. Pax6 is present in the adult corneal epithelium, and we showed that the amount of Pax6 is increased at the migrating front as the epithelium resurfaces the cornea after injury. We also showed that Pax6 controls activity of the transcriptional promoter for the matrix metalloproteinase, gelatinase B (gelB; MMP-9) in cell culture transfection studies. gelB expression is turned on at the migrating epithelial front in the cornea, and it coordinates and effects aspects of epithelial regeneration. We define here two positively acting Pax6 response elements in the gelB promoter. Pax6 binds directly to one of these sites through the paired DNA-binding domain. It binds the second site indirectly by interaction with AP-2alpha, a transcription factor that also exerts control over eye development. Pax6 control of gelB expression was examined in vivo by using a corneal reepithelialization model in mice heterozygous for a Pax6 paired-domain mutation (Sey(+/-)). A reduced Pax6 dosage in these mice resulted in a loss of gelB expression at the migrating epithelial front. This effect was correlated with an increase in inflammation and the rate of reepithelialization, a finding consistent with the phenotype of gelB knockout mice. Together, these data indicate that Pax6 controls activity of the gelB promoter through cooperative interactions with AP-2alpha and support an active role for Pax6 in maintenance and repair of the adult corneal epithelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.