Evidence map›Paper›PMID 14734770›Full record

ArticleJournal of immunology (Baltimore, Md. : 1950)2004

A molecular defect in intracellular lipid signaling in human neutrophils in localized aggressive periodontal tissue damage.

Karsten Gronert, Alpdogan Kantarci, Bruce D Levy, Clary B Clish, Sabine Odparlik, Hatice Hasturk, John A Badwey, Sean P Colgan, Thomas E Van Dyke, Charles N Serhan

Open access · bronzeAbstract read
In one paragraph

Article in Journal of immunology (Baltimore, Md. : 1950), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
5.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. [Gene polymorphisms of cytochrome B-245 alpha chain (Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2022
    Article
  4. Review
  5. Review
  6. DGKα in Neutrophil Biology and Its Implications for Respiratory Diseases.International journal of molecular sciences · 2019
    Review
  7. Lipid mediators in immune regulation and resolution.British journal of pharmacology · 2019
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Inhibition of Corneal Inflammation by the Resolvin E1.Investigative ophthalmology & visual science · 2015
    Article
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  15. Article
  16. Review
  17. Review
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Karsten GronertDepartment of Anesthesiology, Perioperative and Pain Medicine, Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Alpdogan Kantarci
Bruce D Levy
Clary B Clish
Sabine Odparlik
Hatice Hasturk
John A Badwey
Sean P Colgan
Thomas E Van Dyke
Charles N Serhan
Brigham and Women's Hospital · USBoston University · US

Funding

YOGA INDUCED CHANGES IN BRAIN GABA LEVELSM01RR000533 · NCRR · BOSTON UNIVERSITY MEDICAL CAMPUS · PI ANTMAN, KAREN · 1985 to 2008
$19.4M
Novel anti-inflamatory lipid mediatorsP01DE013499 · NIDCR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI SERHAN, CHARLES NICHOLAS · 2000 to 2004
$5.3M
Anti-inflammatory lipids in acute mucosal inflammationK01DK060583 · NIDDK · NEW YORK MEDICAL COLLEGE · PI GRONERT, KARSTEN · 2002 to 2004
$367k
NCRR NIH HHS M01 RR000533NCRR NIH HHS M01-RR00533NIDCR NIH HHS P01 DE013499NIDCR NIH HHS P01-DE13499NIDDK NIH HHS K01 DK060583
6 · The paper itself

Abstract

Host defense mechanisms are impaired in patients with congenital neutrophil (polymorphonuclear neutrophils (PMN)) defects. Impaired PMN chemotaxis is observed in localized aggressive periodontitis (LAP), a familial disorder characterized by destruction of the supporting structures of dentition. In the present studies, we sought evidence for molecular events underlying this aberrant human PMN phenotype. To this end, PMN transendothelial migration and superoxide anion generation were assessed with LAP patients and asymptomatic family members, as well as patients with other chronic mucosal inflammation. PMN from LAP patients showed decreased transmigration across vascular endothelial monolayers (18 +/- 12% of control, n = 4) and increased superoxide anion generation (358 +/- 37%, p = 0.003). Gene expression was analyzed using oligonucleotide microarrays and fluorescence-based kinetic PCR. cDNA microarray and kinetic-PCR analysis revealed diminished RNA expression of leukocyte-type diacylglycerol (DAG) kinase alpha in PMN from LAP patients (4.6 +/- 1.7 relative units, n = 6, p = 0.007) compared with asymptomatic individuals (51 +/- 27 relative units, n = 7). DAG kinase activity was monitored by DAG phosphorylation and individual DAG molecular species were quantified using liquid chromatography and tandem mass spectrometry-based lipidomics. DAG kinase activity was also significantly decreased (73 +/- 2%, p = 0.007) and correlated with increased accumulation of 1,2-diacyl-sn-3-glycerol substrates (p = 0.01). These results implicate defects in both PMN transendothelial migration and PMN DAG kinase alpha signaling as disordered functions in LAP. Moreover, they identify a potential molecular lesion in PMN signal transduction that may account for their aberrant responses and tissue destruction in this disease.

Indexed as

Signal TransductionAdolescentAdultCell Migration InhibitionChemotaxis, LeukocyteChronic DiseaseDiacylglycerol KinaseDiglyceridesEndothelium, VascularFemaleGene Expression ProfilingHumansIntracellular FluidLeukotriene B4MaleNeutrophilsDiacylglycerol KinaseDiglyceridesLeukotriene B4

Identifiers

PMID14734770
PMCPMC4318492
OpenAlexW1923571095

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.