Evidence map›Paper›PMID 15034191›Full record

ArticleEndocrine2004

Effects of prior 5-hydroxytryptamine exposure on rat islet insulin secretory and phospholipase C responses.

Walter S Zawalich, Gregory J Tesz, Kathleen C Zawalich

Abstract read
PubMed Publisher
In one paragraph

Article in Endocrine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. International journal of endocrinology · 2018
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Serotonin regulates glucose-stimulated insulin secretion from pancreatic β cells during pregnancy.Proceedings of the National Academy of Sciences of the United States of America · 2013
    Article
  11. Article
  12. Neurofunctional imaging of β-cell dynamics.Diabetes, obesity & metabolism · 2012
    Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Walter S ZawalichYale University School of Nursing, 100 Church Street South, New Haven, CT 06536-0740, USA. walter.zawalich@yale.edu
Gregory J Tesz
Kathleen C Zawalich
Yale University · US

Funding

PHS HHS 41230
6 · The paper itself

Abstract

Glucose-induced insulin secretion is inhibited by 5-hydroxytryptamine (5HT). In the present studies the specificity of 5HT inhibition of release and the potential biochemical mechanisms involved were investigated. Dose-dependent inhibition of 15 mM glucose-induced secretion was induced by a prior 3 h incubation with 5HT. At the highest 5HT concentration (500 microM) employed, both first and second phase responses to 15 mM glucose were reduced 50-60%. In addition, this level (500 microM) of 5HT virtually abolished 10 mM glucose-induced secretion. In contrast, secretion in response to the protein kinase C activator phorbol 12-myristate 13-acetate (500 nM) was immune to 500 microM 5HT pre-treatment. Glucose usage rates were comparable in both control and 500 microM 5HT-pretreated islets. However, the generation of inositol phosphates and the efflux of 3H-inositol from 3H-inositol-prelabeled islets in response to stimulatory glucose were impaired in parallel with insulin secretion. Based on these observations the following conclusions were reached: (1) 5HT impairs glucose-induced insulin release by altering glucose-induced activation of phospholipase C. (2) Biochemical events distal to phospholipase C remain intact despite this proximal biochemical lesion. (3) Amperometric analysis of 5HT release from 5HT-pretreated islets must take into consideration its profound adverse impact on glucose-induced insulin secretion.

Indexed as

AnimalsEnzyme ActivationGlucoseInositol PhosphatesInsulinInsulin SecretionIslets of LangerhansMaleRatsRats, Sprague-DawleySecretory RateSerotoninTetradecanoylphorbol AcetateType C PhospholipasesGlucoseInositol PhosphatesInsulinSerotoninTetradecanoylphorbol AcetateType C Phospholipases

Identifiers

PMID15034191
OpenAlexW2032352600

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.