Evidence map›Paper›PMID 15039216›Full record

ArticleThe American journal of pathology2004

Increased expression levels of integrin alphavbeta5 on scleroderma fibroblasts.

Yoshihide Asano, Hironobu Ihn, Kenichi Yamane, Masahide Kubo, Kunihiko Tamaki

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in The American journal of pathology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 78 citations in OpenAlex.

  1. Review
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  4. Article
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  9. Epigenetics and systemic sclerosis.Seminars in immunopathology · 2015
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Integrin-mediated regulation of TGFβ in fibrosis.Biochimica et biophysica acta · 2013
    Review
  16. Article
  17. Review
  18. Article
  19. The lens as a model for fibrotic disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yoshihide AsanoDepartment of Dermatology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Hironobu Ihn
Kenichi Yamane
Masahide Kubo
Kunihiko Tamaki
The University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Integrin alphavbeta5 is a receptor for vitronectin, a plasma glycoprotein that is also distributed in extracellular matrix of various tissues. Matrix-bound vitronectin has the potential to stabilize the active form of plasminogen activator inhibitor-1, resulting in the inhibition of the plasmin-mediated pericellular proteolytic cascade. In this study, we compared the levels of alphavbeta5 and matrix-bound vitronectin between normal and scleroderma fibroblasts and investigated the association with fibrosis. We demonstrated that alphavbeta5 was up-regulated on scleroderma fibroblasts. The up-regulated alphavbeta5 contributed to the increase in vitronectin-binding ability in scleroderma fibroblasts, which led to the vitronectin-dependent activation of plasminogen activator inhibitor-1. In immunohistochemistry, the alphav and beta5 subunits were stained strongly on scleroderma fibroblasts and the amount of vitronectin was increased in the pericellular matrix of those cells. The transient overexpression of alphavbeta5 on normal fibroblasts enhanced the human alpha2(I) collagen promoter activity through Sp-1 and Smad3 as well as the vitronectin-dependent plasminogen activator inhibitor-1 activity. This effect on the promoter activity was also observed in the absence of vitronectin and completely disappeared in the presence of anti-alphavbeta5 antibody. These results indicate that the up-regulated alphavbeta5 may contribute to the phenotypical alteration of scleroderma fibroblasts, while at the same time suppressing the plasmin-mediated pericellular proteolytic cascade.

Indexed as

Blotting, NorthernCells, CulturedCollagen Type IDNA-Binding ProteinsFibroblastsHumansImmunohistochemistryIntegrinsMutationPlasminogen Activator Inhibitor 1Precipitin TestsPromoter Regions, GeneticReceptors, VitronectinRNA, MessengerScleroderma, DiffuseSmad3 ProteinCollagen Type IDNA-Binding Proteinsintegrin alphaVbeta5IntegrinsPlasminogen Activator Inhibitor 1Receptors, VitronectinRNA, MessengerSmad3 ProteinSMAD3 protein, humanTrans-ActivatorsTranscription Factor AP-1Vitronectin

Identifiers

PMID15039216
PMCPMC1615355
OpenAlexW2078853486

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.