ArticleThe Biochemical journal2005
An eleven nucleotide section of the 3'-untranslated region is required for perinuclear localization of rat metallothionein-1 mRNA.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Novel Point Mutations in 3'-Untranslated Region of GATA4 Gene Are Associated with Sporadic Non-syndromic Atrial and Ventricular Septal Defects.Current medical science · 2022Article
- The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner.BMC molecular biology · 2015Article
- Zn tolerance of novel Colocasia esculenta metallothionein and its domains in Escherichia coli and tobacco.Journal of plant research · 2012Article
- Computational identification of new structured cis-regulatory elements in the 3'-untranslated region of human protein coding genes.Nucleic acids research · 2012Article
- Fine mapping of interactions between eEF1alpha protein and 3'UTR of metallothionein-1 mRNA.Biochemical and biophysical research communications · 2009Article
- Mutations in the 3'-untranslated region of GATA4 as molecular hotspots for congenital heart disease (CHD).BMC medical genetics · 2007Article
- Article
- Hairpin structure within the 3'UTR of DNA polymerase beta mRNA acts as a post-transcriptional regulatory element and interacts with Hax-1.Nucleic acids research · 2007Article
- Review
- Elongation factor 1alpha binds to the region of the metallothionein-1 mRNA implicated in perinuclear localization--importance of an internal stem-loop.RNA (New York, N.Y.) · 2006Article
- An AU-rich stem-loop structure is a critical feature of the perinuclear localization signal of c-myc mRNA.The Biochemical journal · 2005Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Localization of mRNAs provides a novel mechanism for synthesis of proteins close to their site of function. MT1 (metallothionein-1) is a small, metal-binding protein that is largely cytoplasmic but which can be found in the nucleus. The localization of rat MT1 requires the perinuclear localization of its mRNA by a mechanism dependent on the 3'-UTR (3'-untranslated region). The present study investigates the nature of this mRNA localization signal using Chinese-hamster ovary cells transfected with gene constructs in which either MT1 or the globin coding region is linked to different sequences from the MT1 3'-UTR. Deletion, mutagenesis and antisense oligonucleotide approaches indicate that nt 45-76 of the 3'-UTR, in particular nt 66-76, are required for the localization of either MT1 mRNA or chimaeric transcripts in which a beta-globin coding region is linked to sequences from the MT1 3'-UTR. This section of the 3'-UTR contains a CACC repeat. Two mutations that are predicted to alter the secondary structure of this region also impair localization. Our hypothesis is that the perinuclear localization signal in MT1 mRNA is formed by a combination of the CACC repeat and its structural context.
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