ArticleThe Biochemical journal2005
Phospholipase D activity is essential for actin localization and actin-based motility in Dictyostelium.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Mammalian phospholipase D: Function, and therapeutics.Progress in lipid research · 2020Review
- Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis.PloS one · 2020Article
- Functional interaction of PkcA and PldB regulate aggregation and development in Dictyostelium discoideum.Cellular signalling · 2017Article
- Determination of cell uptake pathways for tumor inhibitor lysyl oxidase propeptide.Molecular oncology · 2016Article
- The molecular basis of leukocyte adhesion involving phosphatidic acid and phospholipase D.The Journal of biological chemistry · 2014Article
- Phospholipase D in cell signaling: from a myriad of cell functions to cancer growth and metastasis.The Journal of biological chemistry · 2014Review
- Activity-dependent dendritic spine shrinkage and growth involve downregulation of cofilin via distinct mechanisms.PloS one · 2014Article
- Phospholipase D (PLD) drives cell invasion, tumor growth and metastasis in a human breast cancer xenograph model.Oncogene · 2013Article
- Regulation of μ-opioid receptors: desensitization, phosphorylation, internalization, and tolerance.Pharmacological reviews · 2013Review
- The exquisite regulation of PLD2 by a wealth of interacting proteins: S6K, Grb2, Sos, WASp and Rac2 (and a surprise discovery: PLD2 is a GEF).Cellular signalling · 2011Review
- Phospholipase D: enzymology, functionality, and chemical modulation.Chemical reviews · 2011Review
- Paxillin and phospholipase D interact to regulate actin-based processes in Dictyostelium discoideum.Eukaryotic cell · 2011Article
- PLD1 rather than PLD2 regulates phorbol-ester-, adhesion-dependent and Fc{gamma}-receptor-stimulated ROS production in neutrophils.Journal of cell science · 2011Article
- Cell density sensing and size determination.Development, growth & differentiation · 2011Review
- Phospholipase D controls Dictyostelium development by regulating G protein signaling.Cellular signalling · 2011Article
- The role of phospholipase D in osteoblast response to titanium surface microstructure.Journal of biomedical materials research. Part A · 2010Article
- Sequential regulation of DOCK2 dynamics by two phospholipids during neutrophil chemotaxis.Science (New York, N.Y.) · 2009Article
- Ca2+ influx and phosphoinositide signalling are essential for the establishment and maintenance of cell polarity in monospores from the red alga Porphyra yezoensis.Journal of experimental botany · 2009Article
- The regulation of cell motility and chemotaxis by phospholipid signaling.Journal of cell science · 2008Review
- Role of lipids and actin in the formation of clathrin-coated pits.Experimental cell research · 2006Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
PLD (phospholipase D) activity catalyses the generation of the lipid messenger phosphatidic acid, which has been implicated in a number of cellular processes, particularly the regulation of membrane traffic. In the present study, we report that disruption of PLD signalling causes unexpectedly profound effects on the actin-based motility of Dictyostelium. Cells in which PLD activity is inhibited by butan-1-ol show a complete loss of actin-based structures, accompanied by relocalization of F-actin into small clusters, and eventually the nucleus, without a visible fall in levels of F-actin. Addition of exogenous phosphatidic acid reverses the effects of butan-1-ol, confirming that these effects are caused by inhibition of PLD. Loss of motility correlates with complete inhibition of endocytosis and a reduction in phagocytosis. Inhibition of PLD caused a major decrease in the synthesis of PtdIns(4,5)P2, which could again be reversed by exogenously applied phosphatidic acid. Thus the essential role of PLD signalling in both motility and endocytosis appears to be mediated directly via regulation of PtdIns(4)P kinase activity. This implies that localized PLD-regulated synthesis of PtdIns(4,5)P2 is essential for Dictyostelium actin function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.