ArticleInvestigative ophthalmology & visual science2005
Targeted deletion of AP-2alpha leads to disruption in corneal epithelial cell integrity and defects in the corneal stroma.
Article in Investigative ophthalmology & visual science, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Genome-wide association study of Fuchs' endothelial corneal dystrophy in the German population.Human genetics · 2025Article
- Snowflake-inspired and blink-driven flexible piezoelectric contact lenses for effective corneal injury repair.Nature communications · 2023Article
- Corneal stromal wound healing: Major regulators and therapeutic targets.The ocular surface · 2021Review
- BMP6 Regulates Corneal Epithelial Cell Stratification by Coordinating Their Proliferation and Differentiation and Is Upregulated in Pterygium.Investigative ophthalmology & visual science · 2020Article
- AP-2ε Expression in Developing Retina: Contributing to the Molecular Diversity of Amacrine Cells.Scientific reports · 2018Article
- Conditional deletion of AP-2β in mouse cranial neural crest results in anterior segment dysgenesis and early-onset glaucoma.Disease models & mechanisms · 2016Article
- AP-2β Is a Downstream Effector of PITX2 Required to Specify Endothelium and Establish Angiogenic Privilege During Corneal Development.Investigative ophthalmology & visual science · 2016Article
- Hemizygous Le-Cre transgenic mice have severe eye abnormalities on some genetic backgrounds in the absence of LoxP sites.PloS one · 2014Article
- Regulation of mouse lens maturation and gene expression by Krüppel-like factor 4.Experimental eye research · 2013Article
- Ocular surface development and gene expression.Journal of ophthalmology · 2013Article
- Overlapping expression patterns and redundant roles for AP-2 transcription factors in the developing mammalian retina.Developmental dynamics : an official publication of the American Association of Anatomists · 2012Article
- Critical role of Klf5 in regulating gene expression during post-eyelid opening maturation of mouse corneas.PloS one · 2012Article
- Conditional disruption of mouse Klf5 results in defective eyelids with malformed meibomian glands, abnormal cornea and loss of conjunctival goblet cells.Developmental biology · 2011Article
- Mouse conjunctival forniceal gene expression during postnatal development and its regulation by Kruppel-like factor 4.Investigative ophthalmology & visual science · 2011Article
- Regulation of corneal epithelial barrier function by Kruppel-like transcription factor 4.Investigative ophthalmology & visual science · 2011Article
- AP-2alpha knockout mice exhibit optic cup patterning defects and failure of optic stalk morphogenesis.Human molecular genetics · 2010Article
- Conditional deletion of Cited2 results in defective corneal epithelial morphogenesis and maintenance.Developmental biology · 2009Article
- Identification of candidate Klf4 target genes reveals the molecular basis of the diverse regulatory roles of Klf4 in the mouse cornea.Investigative ophthalmology & visual science · 2008Article
- Cell autonomous roles for AP-2alpha in lens vesicle separation and maintenance of the lens epithelial cell phenotype.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Article
- Conditional deletion of activating protein 2alpha (AP-2alpha) in the developing retina demonstrates non-cell-autonomous roles for AP-2alpha in optic cup development.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeThe present study was undertaken to create a conditional knockout of AP-2alpha in the corneal epithelium.
methodsA line of mice expressing Cre-recombinase specifically in the early lens placode was crossed with mice in which the AP-2alpha allele is flanked by two loxP sites. The resultant Le-AP-2alpha mutants exhibited a targeted deletion of AP-2alpha in lens placode derivatives, including the differentiating corneal epithelium.
resultsThe Le-AP-2alpha mutant mice were viable and had a normal lifespan. The adult corneal epithelium exhibited a variation in the number of stratified epithelial layers, ranging from 2 to 10 cell layers. A substantial decrease in expression of the cell-cell adhesion molecule, E-cadherin, was observed in all layers of the Le-AP-2alpha mutant corneal epithelium. The basement membrane, or Bowman's layer, was thinner in the mutant cornea and in many regions was discontinuous. These defects corresponded with altered distribution of laminin and entactin, and to a lesser degree, type IV collagen. The Le-AP-2alpha mutant cornea also exhibited stromal defects, including disrupted organization of the collagen lamellae and accumulation of fibroblasts beneath the epithelium that showed increased immunoreactivity for proliferating cell nuclear antigen (PCNA), alpha-smooth muscle actin (alpha-SMA), p-Smad2, and TGF-beta2.
conclusionsIn the absence of AP-2alpha, the corneal epithelium exhibits altered cell adhesion and integrity and defects in its underlying basement membrane. These defects likely caused the alterations in the corneal stroma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.