Evidence map›Paper›PMID 16260592›Full record

ArticleMolecular and cellular biology2005

Interleukin-21 receptor gene induction in human T cells is mediated by T-cell receptor-induced Sp1 activity.

Zheng Wu, Hyoung-Pyo Kim, Hai-Hui Xue, Hong Liu, Keji Zhao, Warren J Leonard

Open access · greenAbstract read
In one paragraph

Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 50 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. The Common Cytokine Receptor γ Chain Family of Cytokines.Cold Spring Harbor perspectives in biology · 2018
    Review
  9. Article
  10. Article
  11. Article
  12. Th17 cells in autoimmune and infectious diseases.International journal of inflammation · 2014
    Review
  13. Article
  14. Article
  15. Inflammatory cytokines in systemic lupus erythematosus.Journal of biomedicine & biotechnology · 2011
    Review
  16. Loss of parity between IL-2 and IL-21 in the NOD Idd3 locus.Proceedings of the National Academy of Sciences of the United States of America · 2009
    Article
  17. Article
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Zheng WuLaboratory of Molecular Immunology, National Heart, Lung, and Blood InstituteNational Institutes of Health, Building 10, Room 7N252, Bethesda, Maryland 20892-1674, USA.
Hyoung-Pyo Kim
Hai-Hui Xue
Hong Liu
Keji Zhao
Warren J Leonard
National Institutes of Health · US

Funding

IL-2 Family Cytokines and their Receptors-- Molecular Regulation via STATsZIAHL005402 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI LEONARD, WARREN J · 2009 to 2025
$26.8M
Genome-wide mapping of histone modificationsZIAHL005801 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI ZHAO, KEJI · 2009 to 2025
$24.0M
Genome-wide mapping of histone modificationsZ01HL005801 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI ZHAO, KEJI · 2003 to 2008
$5.2M
IL-2 Receptors--Molecular Regulation of Receptor ExpressZ01HL005402 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI LEONARD, WARREN J · 1992 to 2008
$2.6M
Intramural NIH HHS
6 · The paper itself

Abstract

Interleukin-21 (IL-21) plays important roles in regulating the immune response. IL-21 receptor (IL-21R) mRNA is expressed at a low level in human resting T cells but is rapidly induced by mitogenic stimulation. We now investigate the basis for IL21R gene regulation in T cells. We found that the -80 to -20 region critically regulates IL-21R promoter activity and corresponds to a major DNase I-hypersensitive site. Electrophoretic mobility shift assays, DNA affinity chromatography followed by mass spectrometry, and chromatin immunoprecipitation assays revealed that Sp1 binds to this region in vitro and in vivo. Moreover, mutation of the Sp1 motif markedly reduced IL-21R promoter activity, and Sp1 small interfering RNAs effectively diminished IL-21R expression in activated T cells. Interestingly, upon T-cell receptor (TCR) stimulation, T cells increased IL-21R expression and Sp1 protein levels while decreasing Sp1 phosphorylation. Moreover, phosphatase inhibitors that increased phosphorylation of Sp1 diminished IL-21R transcription. These data indicate that TCR-induced IL-21R expression is driven by TCR-mediated augmentation of Sp1 protein levels and may partly depend on the dephosphorylation of Sp1.

Indexed as

Gene Expression RegulationAmino Acid MotifsBase SequenceBlotting, WesternChromatin ImmunoprecipitationChromatography, AffinityDeoxyribonuclease IDNA Restriction EnzymesExonsGenes, ReporterHumansInterleukin-21 Receptor alpha SubunitLuciferasesLymphocytesMass SpectrometryModels, GeneticDeoxyribonuclease IDNA Restriction EnzymesIL21R protein, humanInterleukin-21 Receptor alpha SubunitLuciferasesReceptors, Antigen, T-CellReceptors, InterleukinReceptors, Interleukin-21RNA, MessengerRNA, Small InterferingSp1 Transcription FactorSp3 Transcription Factor

Identifiers

PMID16260592
PMCPMC1280258
OpenAlexW2156828576

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.