ArticleDiabetologia2005
Insulin signalling downstream of protein kinase B is potentiated by 5'AMP-activated protein kinase in rat hearts in vivo.
Article in Diabetologia, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Supplementation of scopoletin improves insulin sensitivity by attenuating the derangements of insulin signaling through AMPK.Molecular and cellular biochemistry · 2019Article
- Dual Effects of Metformin on Adipogenic Differentiation of 3T3-L1 Preadipocyte in AMPK-Dependent and Independent Manners.International journal of molecular sciences · 2018Article
- Metformin suppresses adipogenesis through both AMP-activated protein kinase (AMPK)-dependent and AMPK-independent mechanisms.Molecular and cellular endocrinology · 2017Article
- Melatonin-Mediated Intracellular Insulin during 2-Deoxy-d-glucose Treatment Is Reduced through Autophagy and EDC3 Protein in Insulinoma INS-1E Cells.Oxidative medicine and cellular longevity · 2016Article
- Adenosine 5'-monophosphate-activated protein kinase regulates IL-10-mediated anti-inflammatory signaling pathways in macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Chronic AMPK activity dysregulation produces myocardial insulin resistance in the human Arg302Gln-PRKAG2 glycogen storage disease mouse model.EJNMMI research · 2013Article
- Metformin regulates glucose transporter 4 (GLUT4) translocation through AMP-activated protein kinase (AMPK)-mediated Cbl/CAP signaling in 3T3-L1 preadipocyte cells.The Journal of biological chemistry · 2012Article
- AMP-activated protein kinase in the control of cardiac metabolism and remodeling.Current heart failure reports · 2012Review
- Hypoglycemic effects of brassinosteroid in diet-induced obese mice.American journal of physiology. Endocrinology and metabolism · 2012Article
- Defining the contribution of AMP-activated protein kinase (AMPK) and protein kinase C (PKC) in regulation of glucose uptake by metformin in skeletal muscle cells.The Journal of biological chemistry · 2012Article
- Epoxyeicosatrienoic acids and heme oxygenase-1 interaction attenuates diabetes and metabolic syndrome complications.Prostaglandins & other lipid mediators · 2012Review
- Insulin receptor expression and activity in the brains of nondiabetic sporadic Alzheimer's disease cases.International journal of Alzheimer's disease · 2012Article
- AKT signalling in the failing heart.European journal of heart failure · 2011Review
- Regulation of AMPK by the ubiquitin proteasome system.The American journal of pathology · 2011Review
- Adipocyte heme oxygenase-1 induction attenuates metabolic syndrome in both male and female obese mice.Hypertension (Dallas, Tex. : 1979) · 2010Article
- AMP-activated protein kinase antagonizes pro-apoptotic extracellular signal-regulated kinase activation by inducing dual-specificity protein phosphatases in response to glucose deprivation in HCT116 carcinoma.The Journal of biological chemistry · 2010Article
- AMPK exerts dual regulatory effects on the PI3K pathway.Journal of molecular signaling · 2010Article
- Short-term regulation of organic anion transporters.Pharmacology & therapeutics · 2010Review
- The L-4F mimetic peptide prevents insulin resistance through increased levels of HO-1, pAMPK, and pAKT in obese mice.Journal of lipid research · 2009Article
- "Nutritional and chemopreventive anti-cancer agents up-regulate expression of p27Kip1, a cyclin-dependent kinase inhibitor, in mouse JB6 epidermal and human MCF7, MDA-MB-321 and AU565 breast cancer cells".Cancer cell international · 2006Article
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6 authors at 3 institutions in 2 countries.
Funding
Abstract
aims/hypothesis5'AMP-activated protein kinase (AMPK) and insulin stimulate glucose transport in heart and muscle. AMPK acts in an additive manner with insulin to increase glucose uptake, thereby suggesting that AMPK activation may be a useful strategy for ameliorating glucose uptake, especially in cases of insulin resistance. In order to characterise interactions between the insulin- and AMPK-signalling pathways, we investigated the effects of AMPK activation on insulin signalling in the rat heart in vivo.
methodsMale rats (350-400 g) were injected with 1 g/kg 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) or 250 mg/kg metformin in order to activate AMPK. Rats were administered insulin 30 min later and after another 30 min their hearts were removed. The activities and phosphorylation levels of components of the insulin-signalling pathway were subsequently analysed in individual rat hearts.
resultsAICAR and metformin administration activated AMPK and enhanced insulin signalling downstream of protein kinase B in rat hearts in vivo. Insulin-induced phosphorylation of glycogen synthase kinase 3 (GSK3) beta, p70 S6 kinase (p70S6K)(Thr389) and IRS1(Ser636/639) were significantly increased following AMPK activation. To the best of our knowledge, this is the first report of heightened insulin responses of GSK3beta and p70S6K following AMPK activation. In addition, we found that AMPK inhibits insulin stimulation of IRS1-associated phosphatidylinositol 3-kinase activity, and that AMPK activates atypical protein kinase C and extracellular signal-regulated kinase in the heart. CONCLUSIONS/INTERPRETATIONS: Our data are indicative of differential effects of AMPK on the activation of components in the cardiac insulin-signalling pathway. These intriguing observations are critical for characterisation of the crosstalk between AMPK and insulin signalling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.