ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2006
Different effects on fast exocytosis induced by synaptotagmin 1 and 2 isoforms and abundance but not by phosphorylation.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Article
- Expression and Neurotransmitter Association of the Synaptic Calcium Sensor Synaptotagmin in the Avian Auditory Brain Stem.Journal of the Association for Research in Otolaryngology : JARO · 2022Article
- Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion.eLife · 2022Article
- Deconstructing Synaptotagmin-1's Distinct Roles in Synaptic Vesicle Priming and Neurotransmitter Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Brain Acetyl-CoA Production and Phosphorylation of Cytoskeletal Proteins Are Targets of CYP46A1 Activity Modulation and Altered Sterol Flux.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2021Article
- Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and CaeLife · 2021Article
- Biallelic loss of function variants in SYT2 cause a treatable congenital onset presynaptic myasthenic syndrome.American journal of medical genetics. Part A · 2020Article
- Doc2b CaScientific reports · 2019Article
- Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.The Journal of cell biology · 2019Article
- Inositol polyphosphate multikinase deficiency leads to aberrant induction of synaptotagmin-2 in the forebrain.Molecular brain · 2019Article
- The cell adhesion protein CAR is a negative regulator of synaptic transmission.Scientific reports · 2019Article
- SNT-1 Functions as the CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- How does the stimulus define exocytosis in adrenal chromaffin cells?Pflugers Archiv : European journal of physiology · 2018Review
- Doc2B acts as a calcium sensor for vesicle priming requiring synaptotagmin-1, Munc13-2 and SNAREs.eLife · 2017Article
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- Synaptotagmin 2 Is the Fast CaCell reports · 2017Article
- SNARE complex in developmental psychiatry: neurotransmitter exocytosis and beyond.Journal of neural transmission (Vienna, Austria : 1996) · 2016Review
- Extension of Helix 12 in Munc18-1 Induces Vesicle Priming.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Phosphorylation of synaptotagmin-1 controls a post-priming step in PKC-dependent presynaptic plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- PRRT2 Is a Key Component of the Ca(2+)-Dependent Neurotransmitter Release Machinery.Cell reports · 2016Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptotagmins comprise a large protein family, of which synaptotagmin 1 (Syt1) is a Ca2+ sensor for fast exocytosis, and its close relative, synaptotagmin 2 (Syt2), is assumed to serve similar functions. Chromaffin cells express Syt1 but not Syt2. We compared secretion from chromaffin cells from Syt1 null mice overexpressing either Syt isoform. High time-resolution capacitance measurement showed that Syt1 null cells lack the exocytotic phase corresponding to the readily-releasable pool (RRP) of vesicles. Comparison with the amperometric signal confirmed that the missing phase of exocytosis consists of catecholamine-containing vesicles. Overexpression of Syt1 rescued the RRP and increased its size above wild-type values, whereas the size of the slowly releasable pool decreased, indicating that the availability of Syt1 regulates the relative size of the two releasable pools. The RRP was also rescued by Syt2 overexpression, but the kinetics of fusion was slightly slower than in cells expressing Syt1. Biochemical experiments showed that Syt2 has a slightly lower Ca2+ affinity for phospholipid binding than Syt1 because of a difference in the C2A domain. These data constitute evidence for the function of Syt1 and Syt2 as alternative, but not identical, calcium-sensors for RRP fusion. By overexpression of Syt1 mutated in the shared PKC/calcium/calmodulin-dependent kinase phosphorylation site, we show that phorbol esters act independently and upstream of Syt1 to regulate the size of the releasable pools. We conclude that exocytosis from mouse chromaffin cells can be modified by the differential expression of Syt isoforms and by Syt abundance but not by phosphorylation of Syt1.
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