ArticleMolecular genetics and genomics : MGG2006
Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression.
Article in Molecular genetics and genomics : MGG, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 67 citations in OpenAlex.
- Polyamine Metabolism and the DHPS/eIF5A Hypusination Axis: From Metabolic Reprogramming to a Therapeutic Achilles' Heel in Melanoma.Biomolecules · 2026Review
- Identification of hub genes and their expression profiling for predicting buffalo (Bubalus bubalis) semen quality and fertility.Scientific reports · 2023Article
- Role of eIF5A in Mitochondrial Function.International journal of molecular sciences · 2022Review
- Arginine Regulates Zygotic Genome Activation in Porcine Embryos Under Nutrition Restriction.Frontiers in veterinary science · 2022Article
- Eukaryotic translation factor eIF5A contributes to acetic acid tolerance in Saccharomyces cerevisiae via transcriptional factor Ume6p.Biotechnology for biofuels · 2021Article
- eIF5A inhibition influences T cell dynamics in the pancreatic microenvironment of the humanized mouse model of Type 1 Diabetes.Scientific reports · 2019Article
- Modification of translation factor aIF5A from Sulfolobus solfataricus.Extremophiles : life under extreme conditions · 2018Article
- Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins.Scientific reports · 2017Article
- eIF5A facilitates translation termination globally and promotes the elongation of many non polyproline-specific tripeptide sequences.Nucleic acids research · 2017Article
- Exploring polyamines: Functions in embryo/fetal development.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2017Review
- Regulation of gene expression by translation factor eIF5A: Hypusine-modified eIF5A enhances nonsense-mediated mRNA decay in human cells.Translation (Austin, Tex.) · 2017Article
- Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells.Scientific reports · 2016Article
- Posttranslational hypusination of the eukaryotic translation initiation factor-5A regulates Fusarium graminearum virulence.Scientific reports · 2016Article
- Holophytochrome-Interacting Proteins in Physcomitrella: Putative Actors in Phytochrome Cytoplasmic Signaling.Frontiers in plant science · 2016Article
- Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins.The Journal of biological chemistry · 2015Article
- Fertility and polarized cell growth depends on eIF5A for translation of polyproline-rich formins in Saccharomyces cerevisiae.Genetics · 2014Article
- Genome-wide analyses and functional classification of proline repeat-rich proteins: potential role of eIF5A in eukaryotic evolution.PloS one · 2014Article
- Proteomic analysis of rosiglitazone and guggulsterone treated 3T3-L1 preadipocytes.Molecular and cellular biochemistry · 2013Article
- Characterization of a eukaryotic translation initiation factor 5A homolog from Tamarix androssowii involved in plant abiotic stress tolerance.BMC plant biology · 2012Article
- Essential role of eIF5A-1 and deoxyhypusine synthase in mouse embryonic development.Amino acids · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic initiation factor 5A (eIF5A) is the only protein in nature that contains hypusine, an unusual amino acid derived from the modification of lysine by spermidine. Two genes, TIF51A and TIF51B, encode eIF5A in the yeast Saccharomyces cerevisiae. In an effort to understand the structure-function relationship of eIF5A, we have generated yeast mutants by introducing plasmid-borne tif51A into a double null strain where both TIF51A and TIF51B have been disrupted. One of the mutants, tsL102A strain (tif51A L102A tif51aDelta tif51bDelta) exhibits a strong temperature-sensitive growth phenotype. At the restrictive temperature, tsL102A strain also exhibits a cell shape change, a lack of volume change in response to temperature increase and becomes more sensitive to ethanol, a hallmark of defects in the PKC/WSC cell wall integrity pathway. In addition, a striking change in actin dynamics and a complete cell cycle arrest at G1 phase occur in tsL102A cells at restrictive temperature. The temperature-sensitivity of tsL102A strain is due to a rapid loss of mutant eIF5A with the half-life reduced from 6 h at permissive temperature to 20 min at restrictive temperature. Phenylmethyl sulfonylfluoride (PMSF), an irreversible inhibitor of serine protease, inhibited the degradation of mutant eIF5A and suppressed the temperature-sensitive growth arrest. Sorbitol, an osmotic stabilizer that complement defects in PKC/WSC pathways, stabilizes the mutant eIF5A and suppresses all the observed temperature-sensitive phenotypes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.