Evidence map›Paper›PMID 16482468›Full record

ArticleDiabetologia2006

Activation of liver X receptors promotes lipid accumulation but does not alter insulin action in human skeletal muscle cells.

D Cozzone, C Debard, N Dif, N Ricard, E Disse, J Vouillarmet, R Rabasa-Lhoret, M Laville, D Pruneau, J Rieusset and 2 more

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In one paragraph

Article in Diabetologia, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
7.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 58 citations in OpenAlex.

  1. Review
  2. Review
  3. Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
  4. Positioning Metabolism as a Central Player in the Diabetic Heart.Journal of lipid and atherosclerosis · 2020
    Review
  5. Revisiting the Role of LXRs in PUFA Metabolism and Phospholipid Homeostasis.International journal of molecular sciences · 2019
    Review
  6. Tri-Toxicology research · 2016
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. The Randle cycle revisited: a new head for an old hat.American journal of physiology. Endocrinology and metabolism · 2009
    Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

D CozzoneINSERM U449, INRA U1235, Laennec Faculty of Medicine, Claude Bernard University of Lyon, Lyon, France.
C Debard
N Dif
N Ricard
E Disse
J Vouillarmet
R Rabasa-Lhoret
M Laville
D Pruneau
J Rieusset
E Lefai
H Vidal
Inserm · FRUnité de Nutrition Humaine · FRInstitut Alfred Fournier · FRUniversité Claude Bernard Lyon 1 · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aims/hypothesisThe aim of this study was to investigate the effects of liver X receptor (LXR) activation on lipid metabolism and insulin action in human skeletal muscle cells prepared from control subjects and from patients with type 2 diabetes. SUBJECTS AND

methodsCultured myotubes were obtained from muscle biopsies of 11 lean, healthy control subjects and ten patients with type 2 diabetes. The mRNA levels of LXR isoforms and lipogenic genes were estimated by RT-quantitative PCR, and the effects of LXR agonists on insulin action were evaluated by assays of protein kinase B serine 473 phosphorylation and glycogen synthesis.

resultsBoth LXRalpha and LXRbeta were expressed in human skeletal muscle and adipose tissue and there was no difference in their mRNA abundance in tissues from patients with type 2 diabetes compared with control subjects. In cultured muscle cells, LXR activation by T0901317 strongly increased expression of the genes encoding lipogenic enzymes, including sterol regulatory element binding protein 1c, fatty acid synthase and stearoyl-CoA desaturase 1, and also promoted triglyceride accumulation in the presence of a high glucose concentration. Importantly, these effects on lipid metabolism did not affect protein kinase B activation by insulin. Furthermore, LXR agonists did not modify insulin action in muscle cells from patients with type 2 diabetes. CONCLUSIONS/

interpretationThese data suggest that LXR agonists may lead to increased utilisation of lipids and glucose in muscle cells without affecting the mechanism of action of insulin. However, the long-term consequences of triglyceride accumulation in muscle should be evaluated before the development of effective LXR-based therapeutic agents.

Indexed as

AdultBlood GlucoseDNA-Binding ProteinsFatty Acids, NonesterifiedFemaleGlucoseHumansInsulinLiver X ReceptorsMaleMiddle AgedMuscle, SkeletalOrphan Nuclear ReceptorsReceptors, Cytoplasmic and NuclearBlood GlucoseDNA-Binding ProteinsFatty Acids, NonesterifiedGlucoseInsulinLiver X ReceptorsNR1H3 protein, humanOrphan Nuclear ReceptorsReceptors, Cytoplasmic and Nuclear

Identifiers

PMID16482468
OpenAlexW2008963717

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.