ArticleNucleic acids research2006
Different modes and potencies of translational repression by sequence-specific RNA-protein interaction at the 5'-UTR.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Current Practice in Bicistronic IRES Reporter Use: A Systematic Review.International journal of molecular sciences · 2021Pooled it
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- A translational repression reporter assay for the analysis of RNA-binding protein consensus sites.RNA biology · 2023Article
- Review of FRET biosensing and its application in biomolecular detection.American journal of translational research · 2023Review
- PARK2 regulates eIF4B-driven lymphomagenesis.Molecular cancer research : MCR · 2022Article
- BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models.Nature communications · 2021Article
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- Fatty Acid Synthase induced S6Kinase facilitates USP11-eIF4B complex formation for sustained oncogenic translation in DLBCL.Nature communications · 2018Article
- Design of RNA hairpin modules that predictably tune translation in yeast.Synthetic biology (Oxford, England) · 2018Article
- Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.Nucleic acids research · 2017Article
- Enhancing titres of therapeutic viral vectors using the transgene repression in vector production (TRiP) system.Nature communications · 2017Article
- Icariside II, a natural mTOR inhibitor, disrupts aberrant energy homeostasis via suppressing mTORC1-4E-BP1 axis in sarcoma cells.Oncotarget · 2016Article
- The Circadian Protein BMAL1 Regulates Translation in Response to S6K1-Mediated Phosphorylation.Cell · 2015Article
- Protein-responsive ribozyme switches in eukaryotic cells.Nucleic acids research · 2014Article
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- A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector.BMC molecular biology · 2013Article
- Quantitative and simultaneous translational control of distinct mammalian mRNAs.Nucleic acids research · 2013Article
- Kinesin molecular motor Eg5 functions during polypeptide synthesis.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine whether sequence-specific RNA-protein interaction at the 5'-untranslated region (5'-UTR) can potently repress translation in mammalian cells, a bicistronic translational repression assay was developed to permit direct assessment of RNA-protein interaction and translational repression in transiently transfected living mammalian cells. Changes in cap-dependent yellow fluorescent protein (YFP) and internal ribosome entry sequence (IRES)-dependent cyan fluorescent protein (CFP) translation were monitored by fluorescence microscopy. Selective repression of YFP or coordinate repression of both YFP and CFP translation occurred, indicating two distinct modes by which RNA-binding proteins repress translation through the 5'-UTR. Interestingly, a single-stranded RNA-binding protein from Bacillus subtilis, tryptophan RNA-binding attenuation protein (TRAP), showed potent translational repression, dependent on the level of TRAP expression and position of its cognate binding site within the bicistronic reporter transcript. As the first of its class to be examined in mammalian cells, its potency in repression of translation through the 5'-UTR may be a general feature for this class of single-stranded RNA-binding proteins. Finally, a one-hybrid screen based on translational repression through the 5'-UTR identified linkers supporting full-translational repression as well as a range of partial repression by TRAP within the context of a fusion protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.