Evidence map›Paper›PMID 1708093›Full record

ArticleMolecular and cellular biology1991

Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.

J Posada, J Sanghera, S Pelech, R Aebersold, J A Cooper

Open access · greenAbstract readComparative Study
In one paragraph

Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.

0numbers the graph read from it
0cells of the map it votes in
67citing papers in PubMed
10.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

67 citing papers in PubMed, 200 citations in OpenAlex.

  1. Review
  2. Article
  3. The Xenopus cell cycle: an overview.Molecular biotechnology · 2008
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. The beta subunit of CKII negatively regulates Xenopus oocyte maturation.Proceedings of the National Academy of Sciences of the United States of America · 1997
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Cellular expression of MAP 2 kinase in rat brain.Histochemistry and cell biology · 1996
    Article
  19. Article
  20. Article

7 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

J PosadaFred Hutchinson Cancer Research Center, Seattle, Washington 98104.
J Sanghera
S Pelech
R Aebersold
J A Cooper
Fred Hutch Cancer Center · US

Funding

USE OF ANTI-SENSE ONCOGENE EXPRESSIONP01CA028151 · NCI · FRED HUTCHINSON CANCER RESEARCH CENTER · PI NEIMAN, PAUL ERIC · 1985 to 1990
–
NCI NIH HHS CA28151
6 · The paper itself

Abstract

Meiotic maturation of Xenopus and sea star oocytes involves the activation of a number of protein-serine/threonine kinase activities, including a myelin basic protein (MBP) kinase. A 44-kDa MBP kinase (p44mpk) purified from mature sea star oocytes is shown here to be phosphorylated at tyrosine. Antiserum to purified sea star p44mpk was used to identify antigenically related proteins in Xenopus oocytes. Two tyrosine-phosphorylated 42-kDa proteins (p42) were detected with this antiserum in Xenopus eggs. Xenopus p42 chromatographs with MBP kinase activity on a Mono Q ion-exchange column. Tyrosine phosphorylation of Xenopus p42 approximately parallels MBP kinase activity during meiotic maturation. These results suggest that related MBP kinases are activated during meiotic maturation of Xenopus and sea star oocytes. Previous studies have suggested that Xenopus p42 is related to the mitogen-activated protein (MAP) kinases of culture mammalian cells. We have cloned a MAP kinase relative from a Xenopus ovary cDNA library and demonstrate that this clone encodes the Xenopus p42 that is tyrosine phosphorylated during oocyte maturation. Comparison of the sequences of Xenopus p42 and a rat MAP kinase (ERK1) and peptide sequences from sea star p44mpk indicates that these proteins are close relatives. The family members appear to be tyrosine phosphorylated, and activated, in different contexts, with the murine MAP kinase active during the transition from quiescence to the G1 stage of the mitotic cell cycle and the sea star and Xenopus kinases being active during M phase of the meiotic cell cycle.

Indexed as

TyrosineAmino Acid SequenceAnimalsBase SequenceCell CycleCloning, MolecularEnzyme ActivationEpitopesFemaleFibroblastsGene LibraryGlycogen Synthase Kinase 3ImmunoblottingMeiosisMitogensMolecular Sequence DataEpitopesGlycogen Synthase Kinase 3MitogensPeptidesPhosphoproteinsProtein KinasesProtein Serine-Threonine KinasesProtein-Tyrosine KinasesTyrosine

Identifiers

PMID1708093
PMCPMC360021
OpenAlexW1762384317

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.