Evidence mapPaperPMID 18188451Full record

ArticleThe Journal of clinical investigation2008

Claudin-16 and claudin-19 interact and form a cation-selective tight junction complex.

Jianghui Hou, Aparna Renigunta, Martin Konrad, Antonio S Gomes, Eveline E Schneeberger, David L Paul, Siegfried Waldegger, Daniel A Goodenough

Registry-linked trialOpen access · bronzeAbstract read
In one paragraph

Article in The Journal of clinical investigation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06606821 (The Effects of Tirzepatide on Coronary Plaque Lipid Content and Myocardial Microvascular Function in Overweight and Obese People With Coronary Disease - The IDEAL-COR Study), which is not on this map. Cited by 155 papers.

0numbers the graph read from it
0cells of the map it votes in
155citing papers in PubMed
17.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06606821 phase4recruitingstarted 2024, after this paper: background citation

The Effects of Tirzepatide on Coronary Plaque Lipid Content and Myocardial Microvascular Function in Overweight and Obese People With Coronary Disease - The IDEAL-COR Study

Ran2024Enrolled124Registered outcomes23Posted comparisons0ConditionsAtherosclerosis Cardiovascular Disease, Chronic Coronary Artery Disease, Coronary Artery Disease, Coronary Microvascular DysfunctionArmsPlacebo, Tirzepatide
Open the trial in the graph
3 · Its place in the literature

Who cites it

155 citing papers in PubMed, 333 citations in OpenAlex.

  1. Review
  2. Review
  3. Control of renal calcium permeability via a tight junctional claudin switch.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Ion and water permeation through claudin-10b and claudin-15 paracellular channels.Computational and structural biotechnology journal · 2024
    Article
  12. Review
  13. Review
  14. Calcium signalling and transport in the kidney.Nature reviews. Nephrology · 2024
    Review
  15. Review
  16. Article
  17. Article
  18. Review
  19. Article
  20. The role of claudins in homeostasis.Nature reviews. Nephrology · 2023
    Review

95 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Jianghui HouDepartment of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Aparna Renigunta
Martin Konrad
Antonio S Gomes
Eveline E Schneeberger
David L Paul
Siegfried Waldegger
Daniel A Goodenough
Harvard University · USPhilipps University of Marburg · DEMassachusetts General Hospital · USUniversity of Münster · DE

Funding

LENS METABOLIC COOPERATION, GAP JUNCTIONS, &CATARACTR01EY002430 · HARVARD UNIVERSITY (MEDICAL SCHOOL) · 1985 to 2005
$2.8M
IMMUNE FUNCTIONS OF ACCESSORY CELLS IN THE LUNGR01HL036781 · MASSACHUSETTS GENERAL HOSPITAL · 1986 to 2005
$2.3M
MOLECULAR CLONING OF CARDIAC GAP JUNCTION ION CHANNELSR01GM037751 · HARVARD UNIVERSITY (MEDICAL SCHOOL) · 1987 to 2005
$2.3M
PERMEABILITY OF THE LUNG TO WATER-SOLUBLE SOLUTESR01HL025822 · MASSACHUSETTS GENERAL HOSPITAL · 1985 to 2004
$1.9M
Connexins and electrical synapses in the retinaR01EY014127 · HARVARD UNIVERSITY (MEDICAL SCHOOL) · 2004 to 2005
$509k
NEI NIH HHS EY014127NEI NIH HHS EY02430NEI NIH HHS R01 EY002430NEI NIH HHS R01 EY014127NHLBI NIH HHS HL25822NHLBI NIH HHS HL36781NHLBI NIH HHS R01 HL025822NHLBI NIH HHS R01 HL036781NIGMS NIH HHS GM37751NIGMS NIH HHS R01 GM037751
6 · The paper itself

Abstract

Tight junctions (TJs) play a key role in mediating paracellular ion reabsorption in the kidney. Familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) is an inherited disorder caused by mutations in the genes encoding the TJ proteins claudin-16 (CLDN16) and CLDN19; however, the mechanisms underlying the roles of these claudins in mediating paracellular ion reabsorption in the kidney are not understood. Here we showed that in pig kidney epithelial cells, CLDN19 functioned as a Cl(-) blocker, whereas CLDN16 functioned as a Na(+) channel. Mutant forms of CLDN19 that are associated with FHHNC were unable to block Cl(-) permeation. Coexpression of CLDN16 and CLDN19 generated cation selectivity of the TJ in a synergistic manner, and CLDN16 and CLDN19 were observed to interact using several criteria. In addition, disruption of this interaction by introduction of FHHNC-causing mutant forms of either CLDN16 or CLDN19 abolished their synergistic effect. Our data show that CLDN16 interacts with CLDN19 and that their association confers a TJ with cation selectivity, suggesting a mechanism for the role of mutant forms of CLDN16 and CLDN19 in the development of FHHNC.

Indexed as

AnimalsCations, MonovalentCells, CulturedChlorineClaudinsHypercalciuriaImmunoprecipitationIon TransportMagnesiumMembrane ProteinsMutationNephrocalcinosisPermeabilitySodium ChannelsSwineTight JunctionsCations, MonovalentChlorineclaudin 16ClaudinsMagnesiumMembrane ProteinsSodium Channels

Identifiers

PMID18188451
PMCPMC2176193
OpenAlexW2091422963

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.