ArticleMolecular and cellular biology2008
Delineation of the protein module that anchors HMGN proteins to nucleosomes in the chromatin of living cells.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 56 citations in OpenAlex.
- Structural basis of nucleosome recognition by the conserved Dsup and HMGN nucleosome-binding motif.Genes & development · 2025Article
- Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage.Nature communications · 2025Article
- APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons.Nature communications · 2025Article
- Structural basis of nucleosome recognition by the conserved Dsup and HMGN nucleosome-binding motif.bioRxiv : the preprint server for biology · 2025Article
- An evolutionarily distinct Hmgn2 variant influences shape recognition in Medaka Fish.Communications biology · 2024Article
- The role of high mobility group proteins in cellular senescence mechanisms.Frontiers in aging · 2024Review
- Shaking up the silence: consequences of HMGN1 antagonizing PRC2 in the Down syndrome brain.Epigenetics & chromatin · 2022Review
- Article
- Nucleosome Binding by the Lysine Specific Demethylase 1 (LSD1) Enzyme Enables Histone H3 Demethylation.Biochemistry · 2020Article
- Poly(ADP-ribose)-dependent chromatin unfolding facilitates the association of DNA-binding proteins with DNA at sites of damage.Nucleic acids research · 2019Article
- The tardigrade damage suppressor protein binds to nucleosomes and protects DNA from hydroxyl radicals.eLife · 2019Article
- Structural Features of Transcription Factors Associating with Nucleosome Binding.Molecular cell · 2019Article
- HMGN1 and 2 remodel core and linker histone tail domains within chromatin.Nucleic acids research · 2017Article
- Elevated HMGN4 expression potentiates thyroid tumorigenesis.Carcinogenesis · 2017Article
- HMGN proteins modulate chromatin regulatory sites and gene expression during activation of naïve B cells.Nucleic acids research · 2016Article
- Functional interplay between histone H1 and HMG proteins in chromatin.Biochimica et biophysica acta · 2016Review
- Functional compensation among HMGN variants modulates the DNase I hypersensitive sites at enhancers.Genome research · 2015Article
- Growth Cone Localization of the mRNA Encoding the Chromatin Regulator HMGN5 Modulates Neurite Outgrowth.Molecular and cellular biology · 2015Article
- Evolution of high mobility group nucleosome-binding proteins and its implications for vertebrate chromatin specialization.Molecular biology and evolution · 2015Article
- HMGB1 in health and disease.Molecular aspects of medicine · 2014Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Numerous nuclear proteins bind to chromatin by targeting unique DNA sequences or specific histone modifications. In contrast, HMGN proteins recognize the generic structure of the 147-bp nucleosome core particle. HMGNs alter the structure and activity of chromatin by binding to nucleosomes; however, the determinants of the specific interaction of HMGNs with chromatin are not known. Here we use systematic mutagenesis, quantitative fluorescence recovery after photobleaching, fluorescence imaging, and mobility shift assays to identify the determinants important for the specific binding of these proteins to both the chromatin of living cells and to purified nucleosomes. We find that several regions of the protein affect the affinity of HMGNs to chromatin; however, the conserved sequence RRSARLSA, is the sole determinant of the specific interaction of HMGNs with nucleosomes. Within this sequence, each of the 4 amino acids in the R-S-RL motif are the only residues absolutely essential for anchoring HMGN protein to nucleosomes, both in vivo and in vitro. Our studies identify a new chromatin-binding module that specifically recognizes nucleosome cores independently of DNA sequence or histone tail modifications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.