ArticleMolecular genetics and genomics : MGG2008
Gene expression enhancement mediated by the 5' UTR intron of the rice rubi3 gene varied remarkably among tissues in transgenic rice plants.
Article in Molecular genetics and genomics : MGG, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 86 citations in OpenAlex.
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- Size-dependent enhancement of gene expression by Plasmodium 5'UTR introns.Parasites & vectors · 2024Article
- Where the minor things are: a pan-eukaryotic survey suggests neutral processes may explain much of minor intron evolution.Nucleic acids research · 2023Article
- Plant Promoters and Terminators for High-Precision Bioengineering.Biodesign research · 2023Review
- Comparative Analysis of Alternative Splicing in Two Contrasting Apple Cultivars Defense againstInternational journal of molecular sciences · 2022Article
- Genome-Wide Identification and Characterization of UTR-Introns ofInternational journal of molecular sciences · 2020Article
- Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters.Plant biotechnology journal · 2020Article
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- Identification of transcription factors that bind to the 5'-UTR of the barley PHO2 gene.Plant molecular biology · 2020Article
- Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene3 Biotech · 2019Article
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- A novel PR10 promoter from Erianthus arundinaceus directs high constitutive transgene expression and is enhanced upon wounding in heterologous plant systems.Molecular biology reports · 2016Article
- Truncated Ubiquitin 5' Regulatory Region from Erianthus arundinaceus Drives Enhanced Transgene Expression in Heterologous Systems.Molecular biotechnology · 2015Article
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- The intron and 5' distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues.Plant cell reports · 2015Article
- Quantitative trait locus mapping and candidate gene analysis for plant architecture traits using whole genome re-sequencing in rice.Molecules and cells · 2014Article
- Identification, molecular cloning and expression analysis of five RNA-dependent RNA polymerase genes in Salvia miltiorrhiza.PloS one · 2014Article
- The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers.Transgenic research · 2013Article
- Spliceosomal introns in the 5' untranslated region of plant BTL RING-H2 ubiquitin ligases are evolutionary conserved and required for gene expression.BMC plant biology · 2013Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introns are important sequence elements that modulate the expression of genes. Using the GUS reporter gene driven by the promoter of the rice (Oryza sativa L.) polyubiquitin rubi3 gene, we investigated the effects of the 5' UTR intron of the rubi3 gene and the 5' terminal 27 bp of the rubi3 coding sequence on gene expression in stably transformed rice plants. While the intron enhanced GUS gene expression, the 27-bp fused to the GUS coding sequence further augmented GUS expression level, with both varying among different tissues. The intron elevated GUS gene expression mainly at mRNA accumulation level, but also stimulated enhancement at translational level. The enhancement on mRNA accumulation, as determined by realtime quantitative RT-PCR, varied remarkably with tissue type. The augmentation by the intron at translational level also differed by tissue type, but to a lesser extent. On the other hand, the 27-bp fusion further boosted GUS protein yield without affecting mRNA accumulation level, indicating stimulation at translation level, which was also affected by tissue type. The research revealed substantial variation in the magnitudes of intron-mediated enhancement of gene expression (IME) among tissues in rice plants and the importance of using transgenic plants for IME studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.