ArticleBiophysical journal2008
Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
91 citing papers in PubMed, 201 citations in OpenAlex.
- Nuclear and cellular alignment of primary corneal epithelial cells on topography.Journal of biomedical materials research. Part A · 2013Trial
- Dental implant nanotopography for peri-implant tissue stability: soft-tissue sealing, innate immune calibration, and active biointerface regulation.International journal of implant dentistry · 2026Review
- Vertically Aligned Nanopillar Electrodes: Engineered Interfaces for Electrophysiology and Cell-Electrode Coupling.Small methods · 2026Review
- Material-Induced Nuclear Deformation Controls Chromatin Architecture in Adipose Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A vertically integrated system for tracking and assessing cell-cycle-aware phenotypes under confinement.APL bioengineering · 2026Article
- Microtubules Disruption Alters the Cellular Structures and Mechanics Depending on Underlying Chemical Cues.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- 3D Micropatterned Traction Force Microscopy: A Technique to Control 3D Cell Shape While Measuring Cell-Substrate Force Transmission.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization.Nature communications · 2024Article
- The Effects of Biomimetic Surface Topography on Vascular Cells: Implications for Vascular Conduits.Advanced healthcare materials · 2024Review
- Nucleocytoplasmic transport senses mechanical forces independently of cell density in cell monolayers.Journal of cell science · 2024Article
- Late gestation fetal hypothyroidism alters cell cycle regulation across multiple organ systems.BMC veterinary research · 2024Article
- Loss of connectin novex-3 leads to heart dysfunction associated with impaired cardiomyocyte proliferation and abnormal nuclear mechanics.Scientific reports · 2024Article
- Viscoelasticity of diverse biological samples quantified by Acoustic Force Microrheology (AFMR).Communications biology · 2024Article
- Study of the Effect of Cell Prestress on the Cell Membrane Penetration Behavior by Atomic Force Microscopy.Micromachines · 2023Article
- Modeling ATP-mediated endothelial cell elongation on line patterns.Biomechanics and modeling in mechanobiology · 2022Article
- Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAP.Nature communications · 2022Article
- Viscous shaping of the compliant cell nucleus.APL bioengineering · 2022Article
- Article
- 3D-bioprinted microenvironments for sweat gland regeneration.Burns & trauma · 2022Review
- Epigenetic Reprogramming of Tumor-Associated Fibroblasts in Lung Cancer: Therapeutic Opportunities.Cancers · 2021Review
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shape-dependent local differentials in cell proliferation are considered to be a major driving mechanism of structuring processes in vivo, such as embryogenesis, wound healing, and angiogenesis. However, the specific biophysical signaling by which changes in cell shape contribute to cell cycle regulation remains poorly understood. Here, we describe our study of the roles of nuclear volume and cytoskeletal mechanics in mediating shape control of proliferation in single endothelial cells. Micropatterned adhesive islands were used to independently control cell spreading and elongation. We show that, irrespective of elongation, nuclear volume and apparent chromatin decondensation of cells in G1 systematically increased with cell spreading and highly correlated with DNA synthesis (percent of cells in the S phase). In contrast, cell elongation dramatically affected the organization of the actin cytoskeleton, markedly reduced both cytoskeletal stiffness (measured dorsally with atomic force microscopy) and contractility (measured ventrally with traction microscopy), and increased mechanical anisotropy, without affecting either DNA synthesis or nuclear volume. Our results reveal that the nuclear volume in G1 is predictive of the proliferative status of single endothelial cells within a population, whereas cell stiffness and contractility are not. These findings show that the effects of cell mechanics in shape control of proliferation are far more complex than a linear or straightforward relationship. Our data are consistent with a mechanism by which spreading of cells in G1 partially enhances proliferation by inducing nuclear swelling and decreasing chromatin condensation, thereby rendering DNA more accessible to the replication machinery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.