ArticleCell stem cell2008
Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins.
Article in Cell stem cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Glycosylation in Stem Cell Biology.Handbook of experimental pharmacology · 2025Review
- Modeling human trophoblast, the placental epithelium at the maternal fetal interface.Reproduction (Cambridge, England) · 2020Review
- The Glycosylphosphatidylinositol biosynthesis pathway in human diseases.Orphanet journal of rare diseases · 2020Review
- Article
- Targeting RNA helicase DDX3 in stem cell maintenance and teratoma formation.Genes & cancer · 2019Article
- Article
- Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts.Stem cell reports · 2015Article
- Direct Reprogramming of Human Primordial Germ Cells into Induced Pluripotent Stem Cells: Efficient Generation of Genetically Engineered Germ Cells.Stem cells and development · 2015Article
- Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Activin/nodal signaling switches the terminal fate of human embryonic stem cell-derived trophoblasts.The Journal of biological chemistry · 2015Article
- Glycosyl phosphatidylinositol anchor biosynthesis is essential for maintaining epithelial integrity during Caenorhabditis elegans embryogenesis.PLoS genetics · 2015Article
- The genotypic and phenotypic spectrum of PIGA deficiency.Orphanet journal of rare diseases · 2015Article
- Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be?Reproduction (Cambridge, England) · 2014Article
- BMP4 regulation of human trophoblast development.The International journal of developmental biology · 2014Review
- STO Feeder Cells Are Useful for Propagation of Primarily Cultured Human Deciduous Dental Pulp Cells by Eliminating Contaminating Bacteria and Promoting Cellular Outgrowth.Cell medicine · 2013Article
- Generation of glycosylphosphatidylinositol anchor protein-deficient blood cells from human induced pluripotent stem cells.Stem cells translational medicine · 2013Article
- BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ΔNp63+ cytotrophoblast stem cell state.Development (Cambridge, England) · 2013Article
- Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- FGF inhibition directs BMP4-mediated differentiation of human embryonic stem cells to syncytiotrophoblast.Stem cells and development · 2012Article
- The small population of PIG-A mutant cells in myelodysplastic syndromes do not arise from multipotent hematopoietic stem cells.Haematologica · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Pluripotent human embryonic stem (hES) cells can differentiate into various cell types derived from the three embryonic germ layers and extraembryonic tissues such as trophoblasts. The mechanisms governing lineage choices of hES cells are largely unknown. Here, we report that we established two independent hES cell clones lacking a group of cell surface molecules, glycosyl-phosphatidyl-inositol-anchored proteins (GPI-APs). The GPI-AP deficiency in these two hES clones is due to the deficiency in the gene expression of PIG-A (phosphatidyl-inositol-glycan class A), which is required for the first step of GPI synthesis. GPI-AP-deficient hES cells were capable of forming embryoid bodies and initiating cell differentiation into the three embryonic germ layers. However, GPI-AP-deficient hES cells failed to form trophoblasts after differentiation induction by embryoid body formation or by adding exogenous BMP4. The defect in trophoblast formation was due to the lack of GPI-anchored BMP coreceptors, resulting in the impairment of full BMP4 signaling activation in the GPI-AP-deficient hES cells. These data reveal that GPI-AP-enhanced full activation of BMP signaling is required for human trophoblast formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.