ArticleExperimental hematology2009
A KIT juxtamembrane PY567 -directed pathway provides nonredundant signals for erythroid progenitor cell development and stress erythropoiesis.
Article in Experimental hematology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Physiological and regenerative functions of sterile-α motif protein-14 in hematopoiesis.Experimental hematology · 2023Article
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- Sterile α-motif domain requirement for cellular signaling and survival.The Journal of biological chemistry · 2020Article
- Reappraising the role of α5 integrin and the microenvironmental support in stress erythropoiesis.Experimental hematology · 2020Article
- Article
- Mechanisms of erythrocyte development and regeneration: implications for regenerative medicine and beyond.Development (Cambridge, England) · 2018Review
- Dysregulated myelopoiesis and hematopoietic function following acute physiologic insult.Current opinion in hematology · 2018Review
- Direct engagement of the PI3K pathway by mutant KIT dominates oncogenic signaling in gastrointestinal stromal tumor.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia.Developmental cell · 2017Article
- Governing roles for Trib3 pseudokinase during stress erythropoiesis.Experimental hematology · 2017Article
- The spleen microenvironment influences disease transformation in a mouse model of KITScientific reports · 2017Article
- Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis.Journal of cellular and molecular medicine · 2014Article
- Article
- Genetic interaction between Kit and Scl.Blood · 2013Article
- The expression of the glucocorticoid receptor in human erythroblasts is uniquely regulated by KIT ligand: implications for stress erythropoiesis.Stem cells and development · 2012Article
- Reduced ribosomal protein gene dosage and p53 activation in low-risk myelodysplastic syndrome.Blood · 2011Article
- Stress erythropoiesis: new signals and new stress progenitor cells.Current opinion in hematology · 2011Review
- Article
- A conserved threonine residue in the juxtamembrane domain of the XA21 pattern recognition receptor is critical for kinase autophosphorylation and XA21-mediated immunity.The Journal of biological chemistry · 2010Article
- Role of stem cell factor in the reactivation of human fetal hemoglobin.Mediterranean journal of hematology and infectious diseases · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveKITL/KIT can elicit diverse sets of signals within lymphoid, myeloid, mast, and erythroid lineages, and exert distinct effects on growth, survival, migration, adhesion, and secretory responses. Presently, we have applied a PY-mutant allele knockin approach to specifically assess possible roles for KIT-PY567 and KIT-PY719 sites, and coupled pathways, during erythropoiesis. MATERIALS AND
methodsMouse models used to investigate this problem include those harboring knocked-in KIT(Y567F/Y567F), KIT(Y569F/Y569F), KIT(Y719F,Y719F), and KIT(Y567F/Y567F:Y569F/Y569F) alleles. The erythron was stressed by myelosuppression using 5-fluorouracil, and by phenylhydrazine-induced hemolysis. In addition, optimized systems for ex vivo analyses of bone marrow and splenic erythropoiesis were employed to more directly analyze possible stage-specific effects on erythroid cell growth, survival, development and KIT signaling events.
resultsIn Kit(Y567F/Y567F) mice, steady-state erythropoiesis was unperturbed while recovery from anemia due to 5-fluorouracil or phenylhydrazine was markedly impaired. Deficiencies in erythroid progenitor expansion occurred both in the bone marrow and the spleen. Responses to chronic erythropoietin dosing were also compromised. Ex vivo, Kit(Y567F/Y567F) (pro)erythroblast development was skewed from a Kit(pos)CD71(high) stage toward a subsequent Kit(neg)CD71(high) compartment. Proliferation and, to an extent, survival capacities were also compromised. Similar stage-specific defects existed for erythroid progenitors from Kit(Y567F/Y567F:Y569F/Y569F) but not KIT(Y719F/Y719F) mice. Kit(Y567F/Y567F) erythroblasts were used further to analyze KIT-PY567-dependent signals. MEK-1,2/ERK-1,2 signaling was unaffected while AKT, p70S6K, and especially JNK2/p54 pathways were selectively attenuated.
conclusionsNonredundant KIT-PY567-directed erythroblast-intrinsic signals are selectively critical for stress erythropoiesis. Investigations also add to an understanding of how KIT directs distinct outcomes among diverse progenitors and lineages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.