ArticleTraffic (Copenhagen, Denmark)2009
Control of granule mobility and exocytosis by Ca2+ -dependent formation of F-actin in pancreatic duct epithelial cells.
Article in Traffic (Copenhagen, Denmark), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 12 citations in OpenAlex.
- Contributions of protein kinases and β-arrestin to termination of protease-activated receptor 2 signaling.The Journal of general physiology · 2016Article
- Directional bleb formation in spherical cells under temperature gradient.Biophysical journal · 2015Article
- Noradrenaline upregulates T-type calcium channels in rat pinealocytes.The Journal of physiology · 2015Article
- Real-time imaging of exocytotic mucin release and swelling in Calu-3 cells using acridine orange.Methods (San Diego, Calif.) · 2014Article
- Epithelial monolayer culture system for real-time single-cell analyses.Physiological reports · 2014Article
- Article
- Actin cytoskeleton controls movement of intracellular organelles in pancreatic duct epithelial cells.Cell calcium · 2012Article
- Distinct role of rab27a in granule movement at the plasma membrane and in the cytosol of NK cells.PloS one · 2010Article
- Cyclic AMP potentiates Ca2+-dependent exocytosis in pancreatic duct epithelial cells.The Journal of general physiology · 2010Article
- Individual calcium syntillas do not trigger spontaneous exocytosis from nerve terminals of the neurohypophysis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Elevation of intracellular Ca(2+) concentration ([Ca(2+)](i)) triggers exocytosis of secretory granules in pancreatic duct epithelia. In this study, we find that the signal also controls granule movement. Motions of fluorescently labeled granules stopped abruptly after a [Ca(2+)](i) increase, kinetically coincident with formation of filamentous actin (F-actin) in the whole cytoplasm. At high resolution, the new F-actin meshwork was so dense that cellular structures of granule size appeared physically trapped in it. Depolymerization of F-actin with latrunculin B blocked both the F-actin formation and the arrest of granules. Interestingly, when monitored with total internal reflection fluorescence microscopy, the immobilized granules still moved slowly and concertedly toward the plasma membrane. This group translocation was abolished by blockers of myosin. Exocytosis measured by microamperometry suggested that formation of a dense F-actin meshwork inhibited exocytosis at small Ca(2+) rises <1 microm. Larger [Ca(2+)](i) rises increased exocytosis because of the co-ordinate translocation of granules and fusion to the membrane. We propose that the Ca(2+)-dependent freezing of granules filters out weak inputs but allows exocytosis under stronger inputs by controlling granule movements.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.