Evidence map›Paper›PMID 19203376›Full record

ArticleBMC genomics2009

MytiBase: a knowledgebase of mussel (M. galloprovincialis) transcribed sequences.

Paola Venier, Cristiano De Pittà, Filippo Bernante, Laura Varotto, Barbara De Nardi, Giuseppe Bovo, Philippe Roch, Beatriz Novoa, Antonio Figueras, Alberto Pallavicini and 1 more

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
15.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 145 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 3 countries.

Paola VenierDepartment of Biology, University of Padova, Via U Bassi 58/B, Padova, Italy. paola.venier@unipd.it
Cristiano De Pittà
Filippo Bernante
Laura Varotto
Barbara De Nardi
Giuseppe Bovo
Philippe Roch
Beatriz Novoa
Antonio Figueras
Alberto Pallavicini
Gerolamo Lanfranchi
University of Padua · ITInstituto de Investigacións Mariñas · ESIstituto Zooprofilattico Sperimentale delle Venezie · ITUniversité de Montpellier · FRUniversity of Trieste · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAlthough bivalves are among the most studied marine organisms due to their ecological role, economic importance and use in pollution biomonitoring, very little information is available on the genome sequences of mussels. This study reports the functional analysis of a large-scale Expressed Sequence Tag (EST) sequencing from different tissues of Mytilus galloprovincialis (the Mediterranean mussel) challenged with toxic pollutants, temperature and potentially pathogenic bacteria.

resultsWe have constructed and sequenced seventeen cDNA libraries from different Mediterranean mussel tissues: gills, digestive gland, foot, anterior and posterior adductor muscle, mantle and haemocytes. A total of 24,939 clones were sequenced from these libraries generating 18,788 high-quality ESTs which were assembled into 2,446 overlapping clusters and 4,666 singletons resulting in a total of 7,112 non-redundant sequences. In particular, a high-quality normalized cDNA library (Nor01) was constructed as determined by the high rate of gene discovery (65.6%). Bioinformatic screening of the non-redundant M. galloprovincialis sequences identified 159 microsatellite-containing ESTs. Clusters, consensuses, related similarities and gene ontology searches have been organized in a dedicated, searchable database http://mussel.cribi.unipd.it.

conclusionWe defined the first species-specific catalogue of M. galloprovincialis ESTs including 7,112 unique transcribed sequences. Putative microsatellite markers were identified. This annotated catalogue represents a valuable platform for expression studies, marker validation and genetic linkage analysis for investigations in the biology of Mediterranean mussels.

Indexed as

Databases, GeneticExpressed Sequence TagsKnowledge BasesAnimalsCluster AnalysisComputational BiologyGene LibraryGenetic MarkersMicrosatellite RepeatsMytilusSequence Analysis, DNAUser-Computer InterfaceGenetic Markers

Identifiers

PMID19203376
PMCPMC2657158
OpenAlexW2113353869

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.