Evidence map›Paper›PMID 19204083›Full record

ArticleMolecular and cellular biology2009

Physical interaction between TBX5 and MEF2C is required for early heart development.

Tushar K Ghosh, Fei Fei Song, Elizabeth A Packham, Sarah Buxton, Thelma E Robinson, Jonathan Ronksley, Tim Self, Andrew J Bonser, J David Brook

Open access · greenAbstract read
In one paragraph

Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed, 2 pooled it
4.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 2 syntheses or guidelines pooled it, 133 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Review
  10. Review
  11. The molecular mechanisms of cardiac development and related diseases.Signal transduction and targeted therapy · 2024
    Review
  12. Review
  13. Article
  14. Cardiac Transcription Factors and Regulatory Networks.Advances in experimental medicine and biology · 2024
    Review
  15. Article
  16. Article
  17. Article
  18. Direct Reprogramming of Cardiac Fibroblasts to Repair the Injured Heart.Journal of cardiovascular development and disease · 2021
    Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Tushar K GhoshInstitute of Genetics, School of Biology, University of Nottingham, Queen's Medical Centre, Nottingham, United Kingdom.
Fei Fei Song
Elizabeth A Packham
Sarah Buxton
Thelma E Robinson
Jonathan Ronksley
Tim Self
Andrew J Bonser
J David Brook
Institute of Genetics · HUUniversity of Nottingham · GB

Funding

British Heart Foundation RG/07/010/23676Wellcome Trust
6 · The paper itself

Abstract

TBX5 is a transcription factor which plays important roles in the development of the heart and upper limbs. Mutations in this gene produce the inherited disorder Holt-Oram syndrome. Here, we report a physical interaction between TBX5 and MEF2C leading to a synergistic activation of the alpha-cardiac myosin heavy chain (MYH6). Mutants of TBX5, TBX5G80R, and TBX5R279X that produce severe cardiac phenotypes impair the synergy. Using fluorescence resonance energy transfer, we demonstrate the interaction of TBX5 and MEF2C in living cells. We also show that they physically associate through their DNA-binding domains to form a complex on the MYH6 promoter. Morpholino-mediated knockdowns of Tbx5 and Mef2c in zebrafish suggest that the genetic interaction of these proteins is not only required for MYH6 expression but also essential for the early stages of heart development and survival. This is the first report of a functional interaction between a T-box protein and a MADS box factor that may be crucial in cardiomyocyte differentiation.

Indexed as

AnimalsCardiac MyosinsCell LineEmbryo, NonmammalianHeartHumansMutation, MissenseMyogenic Regulatory FactorsMyosin Heavy ChainsPromoter Regions, GeneticRatsT-Box Domain ProteinsTransfectionZebrafishCardiac MyosinsMYH6 protein, humanMyogenic Regulatory FactorsMyosin Heavy ChainsT-Box Domain ProteinsT-box transcription factor 5

Identifiers

PMID19204083
PMCPMC2663302
OpenAlexW2164511316

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.