Evidence map›Paper›PMID 19208690›Full record

ArticleBrain : a journal of neurology2009

Myelin-mediated inhibition of oligodendrocyte precursor differentiation can be overcome by pharmacological modulation of Fyn-RhoA and protein kinase C signalling.

Alexandra S Baer, Yasir A Syed, Sung Ung Kang, Dieter Mitteregger, Raluca Vig, Charles Ffrench-Constant, Robin J M Franklin, Friedrich Altmann, Gert Lubec, Mark R Kotter

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain : a journal of neurology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.

0numbers the graph read from it
0cells of the map it votes in
106citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

106 citing papers in PubMed, 206 citations in OpenAlex.

  1. Review
  2. The role of type I collagen in white matter injury.Anatomical science international · 2026
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  19. Growth arrest specific protein 6 alleviated white matter injury after experimental ischemic stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024
    Article
  20. Review

46 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Alexandra S BaerDepartment of Neurosurgery, Medical University Vienna, Vienna, Austria.
Yasir A Syed
Sung Ung Kang
Dieter Mitteregger
Raluca Vig
Charles Ffrench-Constant
Robin J M Franklin
Friedrich Altmann
Gert Lubec
Mark R Kotter
Medical University of Vienna · ATBOKU University · ATUniversity of Cambridge · GBUniversity of Edinburgh · GB

Funding

Medical Research Council G0300723Medical Research Council G0800784
6 · The paper itself

Abstract

Failure of oligodendrocyte precursor cell (OPC) differentiation contributes significantly to failed myelin sheath regeneration (remyelination) in chronic demyelinating diseases. Although the reasons for this failure are not completely understood, several lines of evidence point to factors present following demyelination that specifically inhibit differentiation of cells capable of generating remyelinating oligodendrocytes. We have previously demonstrated that myelin debris generated by demyelination inhibits remyelination by inhibiting OPC differentiation and that the inhibitory effects are associated with myelin proteins. In the present study, we narrow down the spectrum of potential protein candidates by proteomic analysis of inhibitory protein fractions prepared by CM and HighQ column chromatography followed by BN/SDS/SDS-PAGE gel separation using Nano-HPLC-ESI-Q-TOF mass spectrometry. We show that the inhibitory effects on OPC differentiation mediated by myelin are regulated by Fyn-RhoA-ROCK signalling as well as by modulation of protein kinase C (PKC) signalling. We demonstrate that pharmacological or siRNA-mediated inhibition of RhoA-ROCK-II and/or PKC signalling can induce OPC differentiation in the presence of myelin. Our results, which provide a mechanistic link between myelin, a mediator of OPC differentiation inhibition associated with demyelinating pathologies and specific signalling pathways amenable to pharmacological manipulation, are therefore of significant potential value for future strategies aimed at enhancing CNS remyelination.

Indexed as

1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAnimalsAnimals, NewbornCarbazolesCell DifferentiationDemyelinating DiseasesElectrophoresis, Polyacrylamide GelIndolesMaleimidesMyelin SheathNerve RegenerationOligodendrogliaProtein Kinase C-alphaProto-Oncogene Proteins c-fynRatsRats, Sprague-Dawley1-(5-Isoquinolinesulfonyl)-2-Methylpiperazinebisindolylmaleimide IXCarbazolesfasudilFyn protein, ratGo 6976IndolesMaleimidesProtein Kinase C-alphaProto-Oncogene Proteins c-fynrhoA GTP-Binding Proteinrho-Associated KinasesRNA, Small Interfering

Identifiers

PMID19208690
PMCPMC2640211
OpenAlexW2159729185

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.