ArticleMolecular and cellular biology2009
Dax1 binds to Oct3/4 and inhibits its transcriptional activity in embryonic stem cells.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 73 citations in OpenAlex.
- Genotype-phenotype correlations in specific granule deficiency: loss of DNA-binding ability and impaired nuclear localization cause severe manifestations due to the c.655_665del CEBPE variant.Clinical and experimental immunology · 2025Article
- Screening for DAX1/EWS-FLI1 functional inhibitors identified dihydroorotate dehydrogenase as a therapeutic target for Ewing's sarcoma.Cancer medicine · 2023Article
- Temporal Gene Expression and DNA Methylation during Embryonic Stem Cell Derivation.Cell journal · 2018Article
- Network Features and Dynamical Landscape of Naive and Primed Pluripotency.Biophysical journal · 2018Article
- Baf53a is involved in survival of mouse ES cells, which can be compensated by Baf53b.Scientific reports · 2017Article
- Ground rules of the pluripotency gene regulatory network.Nature reviews. Genetics · 2017Review
- The Pluripotency Factor NANOG Binds to GLI Proteins and Represses Hedgehog-mediated Transcription.The Journal of biological chemistry · 2016Article
- ETS-related transcription factors ETV4 and ETV5 are involved in proliferation and induction of differentiation-associated genes in embryonic stem (ES) cells.The Journal of biological chemistry · 2015Article
- Nr0b1 is a negative regulator of Zscan4c in mouse embryonic stem cells.Scientific reports · 2015Article
- Dax1 and Nanog act in parallel to stabilize mouse embryonic stem cells and induced pluripotency.Nature communications · 2014Article
- Minireview: role of orphan nuclear receptors in cancer and potential as drug targets.Molecular endocrinology (Baltimore, Md.) · 2014Review
- Review
- The orphan nuclear receptors at their 25-year reunion.Journal of molecular endocrinology · 2013Review
- Minireview: the diverse roles of nuclear receptors in the regulation of embryonic stem cell pluripotency.Molecular endocrinology (Baltimore, Md.) · 2013Review
- Differential role for transcription factor Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells.The Journal of biological chemistry · 2013Article
- Dax1 associates with Esrrb and regulates its function in embryonic stem cells.Molecular and cellular biology · 2013Article
- Article
- Embryonic stem cell interactomics: the beginning of a long road to biological function.Stem cell reviews and reports · 2012Review
- Role of nuclear receptor coactivator 3 (Ncoa3) in pluripotency maintenance.The Journal of biological chemistry · 2012Article
- Role of SF-1 and DAX-1 during differentiation of P19 cells by retinoic acid.Journal of cellular physiology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor Oct3/4 is an indispensable factor in the self-renewal of ES cells. In this study, we searched for a protein that would interact with Oct3/4 in ES cells and identified an orphan nuclear hormone receptor, Dax1. The association of Dax1 with Oct3/4 was mediated through the POU-specific domain of Oct3/4. Ectopic expression of Dax1 inhibited Oct3/4-mediated activation of an artificial Oct3/4-responsive promoter. Expression of Dax1 in ES cells also reduced the activities of Nanog and Rex1 promoters, while knockdown of Dax1 increased these activities. Pulldown and gel shift assays revealed that the interaction of Dax1 with Oct3/4 abolished the DNA binding activity of Oct3/4. Chromatin immunoprecipitation assay results showed that Dax1 inhibited Oct3/4 binding to the promoter/enhancer regions of Oct3/4 and Nanog. Furthermore, overexpression of Dax1 resulted in ES cell differentiation. Taken together, these data suggest that Dax1, a novel molecule interacting with Oct3/4, functions as a negative regulator of Oct3/4 in ES cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.