Evidence mapPaperPMID 19587356Full record

ArticleDiabetes2009

Effect of the monocyte chemoattractant protein-1/CC chemokine receptor 2 system on nephrin expression in streptozotocin-treated mice and human cultured podocytes.

Elena Tarabra, Sara Giunti, Federica Barutta, Gennaro Salvidio, Davina Burt, Giacomo Deferrari, Roberto Gambino, Daniela Vergola, Silvia Pinach, Paolo Cavallo Perin and 2 more

Registry-linked trialOpen access · hybridAbstract read
In one paragraph

Article in Diabetes, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01847313 (Phase 3 Study of the Effect of Glucagon-like-peptide 1), which is not on this map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01847313 phase3completedstarted 2013, after this paper: background citation

Phase 3 Study of the Effect of Glucagon-like-peptide 1 (GLP-1) Receptor Agonism on Renal Outcomes in Humans With Diabetic Kidney Disease

Ran2013Enrolled20Registered outcomes6Posted comparisons0ConditionsDiabetic Kidney DiseaseArmsliraglutide
Open the trial in the graph
3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 119 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Mechanisms of podocyte injury and implications for diabetic nephropathy.Clinical science (London, England : 1979) · 2022
    Article
  11. Molecular Mechanisms of Kidney Injury and Repair.International journal of molecular sciences · 2022
    Review
  12. Article
  13. The role of inflammation in diabetic kidney disease.The Korean journal of internal medicine · 2021
    Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Review
  20. The Role of Chemokines and Chemokine Receptors in Diabetic Nephropathy.International journal of molecular sciences · 2020
    Review

4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Elena TarabraDiabetic Nephropathy Laboratory, Department of Internal Medicine, University of Turin, Italy.
Sara Giunti
Federica Barutta
Gennaro Salvidio
Davina Burt
Giacomo Deferrari
Roberto Gambino
Daniela Vergola
Silvia Pinach
Paolo Cavallo Perin
Giovanni Camussi
Gabriella Gruden
University of Turin · ITUniversity of Genoa · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveMonocyte chemoattractant protein-1 (MCP-1), a chemokine binding to the CC chemokine receptor 2 (CCR2) and promoting monocyte infiltration, has been implicated in the pathogenesis of diabetic nephropathy. To assess the potential relevance of the MCP-1/CCR2 system in the pathogenesis of diabetic proteinuria, we studied in vitro if MCP-1 binding to the CCR2 receptor modulates nephrin expression in cultured podocytes. Moreover, we investigated in vivo if glomerular CCR2 expression is altered in kidney biopsies from patients with diabetic nephropathy and whether lack of MCP-1 affects proteinuria and expression of nephrin in experimental diabetes. RESEARCH DESIGN AND

methodsExpression of nephrin was assessed in human podocytes exposed to rh-MCP-1 by immunofluorescence and real-time PCR. Glomerular CCR2 expression was studied in 10 kidney sections from patients with overt nephropathy and eight control subjects by immunohistochemistry. Both wild-type and MCP-1 knockout mice were made diabetic with streptozotocin. Ten weeks after the onset of diabetes, albuminuria and expression of nephrin, synaptopodin, and zonula occludens-1 were examined by immunofluorescence and immunoblotting.

resultsIn human podocytes, MCP-1 binding to the CCR2 receptor induced a significant reduction in nephrin both mRNA and protein expression via a Rho-dependent mechanism. The MCP-1 receptor, CCR2, was overexpressed in the glomerular podocytes of patients with overt nephropathy. In experimental diabetes, MCP-1 was overexpressed within the glomeruli and the absence of MCP-1 reduced both albuminuria and downregulation of nephrin and synaptopodin.

conclusionsThese findings suggest that the MCP-1/CCR2 system may be relevant in the pathogenesis of proteinuria in diabetes.

Indexed as

AnimalsBiopsyCells, CulturedChemokine CCL2Diabetes Mellitus, ExperimentalDiabetic NephropathiesDown-RegulationHumansIn Vitro TechniquesMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMicrofilament ProteinsPhosphoproteinsPodocytesCCL2 protein, humanCcl2 protein, mouseChemokine CCL2Membrane ProteinsMicrofilament ProteinsnephrinPhosphoproteinsRecombinant Proteinsrho-Associated KinasesRNA, MessengerSYNPO protein, humanTJP1 protein, humanTjp1 protein, mouseZonula Occludens-1 Protein

Identifiers

PMID19587356
PMCPMC2731530
OpenAlexW2113583039

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.