Evidence mapPaperPMID 19672102Full record

ArticleJournal of vascular research2010

Protein expression in vascular endothelial cells obtained from human peripheral arteries and veins.

Annemarie E Silver, Demetra D Christou, Anthony J Donato, Stacy D Beske, Kerrie L Moreau, Katherine A Magerko, Douglas R Seals

2 registry-linked trialsOpen access · bronzeAbstract readComparative Study
In one paragraph

Article in Journal of vascular research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
1.1field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05478707 phase2recruitingstarted 2023, after this paper: background citation

Therapeutic Strategies for Microvascular Dysfunction in Type 1 Diabetes

Ran2023Enrolled47Registered outcomes5Posted comparisons0ConditionsDiabetes Mellitus, Type 1, Endothelial DysfunctionArmsDulaglutide, Placebo
Open the trial in the graph
NCT03109795 phase4terminatednot on this mapstarted 2017, after this paper: background citation

Anxiety-mediated Impairments in Large Elastic Artery Function and the Autonomic Nervous System

TypeinterventionalSponsorUniversity of IowaRan2017 to 2023Enrolled35ConditionsAnxiety, HypertensionArmsClonidine Pill, Hydrochlorothiazide
3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 43 citations in OpenAlex.

  1. Vascular Function and Uric Acid-Lowering in Stage 3 CKD.Journal of the American Society of Nephrology : JASN · 2017
    Trial
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  5. Article
  6. Reduced ATHypertension (Dallas, Tex. : 1979) · 2025
    Article
  7. Impaired microvascular insulin-dependent dilation in women with a history of gestational diabetes.American journal of physiology. Heart and circulatory physiology · 2024
    Article
  8. Article
  9. Antecubital venous endothelial ETB receptor protein expression is preserved with aging in men.American journal of physiology. Heart and circulatory physiology · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Altered endothelial ETAmerican journal of physiology. Heart and circulatory physiology · 2020
    Article
  14. Endothelin-1-induced endothelial microvesicles impair endothelial cell function.Journal of applied physiology (Bethesda, Md. : 1985) · 2020
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  15. Article
  16. Article
  17. Comparative Transcriptomics ofJACC. Basic to translational science · 2019
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Annemarie E SilverDepartment of Integrative Physiology, University of Colorado, Boulder, CO 80309,USA.
Demetra D Christou
Anthony J Donato
Stacy D Beske
Kerrie L Moreau
Katherine A Magerko
Douglas R Seals
University of Colorado Boulder · USUniversity of Colorado System · US

Funding

ZOPOLRESTAT PHARMACOKINETICS IN DIABETICS WITH VARYING RENAL FUNCTIONM01RR000051 · UNIVERSITY OF COLORADO DENVER · 1985 to 2005
$37.2M
SYMPATHETIC NERVOUS SYSTEM ACTIVITY &HUMAN AGINGR01AG006537 · UNIVERSITY OF COLORADO AT BOULDER · 1986 to 2005
$2.1M
UCHSC AGING TRAINING GRANTT32AG000279 · UNIVERSITY OF COLORADO DENVER · 2001 to 2025
$2.0M
SODIUM RESTRICTION &ARTERIAL COMPLIANCE IN OLDER HUMANSR01AG013038 · UNIVERSITY OF COLORADO AT BOULDER · 1996 to 2003
$1.6M
MECHANISMS OF CARDIOVASCULAR DISEASE AND GENE THERAPYT32HL007851 · UNIVERSITY OF COLORADO AT BOULDER · 1996 to 2005
$1.1M
HRT, SERMs and LEG BLOOD FLOW IN POSTMENOPAUSAL WOMENR01AG022241 · UNIVERSITY OF COLORADO AT BOULDER · 2004 to 2005
$743k
Human Aging, Exercise &FMD: Translational PhysiologyR37AG013038 · UNIVERSITY OF COLORADO AT BOULDER · 2004 to 2005
$586k
NCRR NIH HHS M01 RR000051NCRR NIH HHS RR00051NHLBI NIH HHS HL007851NHLBI NIH HHS T32 HL007851NIA NIH HHS AG000279NIA NIH HHS AG006537NIA NIH HHS AG013038NIA NIH HHS AG022241NIA NIH HHS AG029337NIA NIH HHS K01 AG029337NIA NIH HHS R01 AG006537NIA NIH HHS R01 AG013038NIA NIH HHS R01 AG022241NIA NIH HHS R37 AG013038NIA NIH HHS T32 AG000279
6 · The paper itself

Abstract

Studying molecular mechanisms of vascular endothelial function in humans is difficult in part because of limited access to arteries. Access to peripheral veins is more practical. We determined if differences in protein expression of endothelial cells (EC) collected from a peripheral artery are reflected in measurements made on EC obtained from peripheral veins. EC were collected from the brachial artery and an antecubital vein of 106 healthy adults (60 men and 46 women, age 18-77 years). Quantitative immunofluorescence was used to measure protein expression of endothelial nitric oxide synthase (eNOS), Ser-1177 phosphorylated eNOS, manganese superoxide dismutase, nitrotyrosine, xanthine oxidase and nuclear factor-kappaB p65. Protein expression in EC obtained from brachial artery and antecubital vein sampling was moderately to strongly related (r = 0.59-0.81, all p < 0.0001, mean r = 0.70). Moreover, differences between subgroups in the lowest and highest tertiles of protein expression in EC obtained from arterial samples were consistently reflected in EC obtained from venous collections. These findings indicate that interindividual and group differences in expression of several proteins involved in nitric oxide production, oxidant production, antioxidant defense and inflammatory signaling in EC obtained from brachial artery sampling are consistently reflected in EC obtained from venous samples. Thus, EC collected from peripheral veins may provide a useful surrogate for EC obtained from arteries for measurements of EC protein expression in humans.

Indexed as

AdolescentAdultAgedBrachial ArteryEndothelial CellsFemaleFluorescent Antibody TechniqueHumansLinear ModelsMaleMicroscopy, FluorescenceMiddle AgedNitric Oxide Synthase Type IIIPhosphorylationProteinsSerine3-nitrotyrosineNitric Oxide Synthase Type IIINOS3 protein, humanProteinsRELA protein, humanSerineSuperoxide DismutaseTranscription Factor RelATyrosineXanthine Oxidase

Identifiers

PMID19672102
PMCPMC2855282
OpenAlexW2065475382

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.